Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday; Matthew N. Van Ert; Tatum S. Simonson; David M. Wagner; Leo J. Kenefic; Christopher J. Allender; Paul Keim

doi:10.1128/JCM.43.4.1995-1997.2005

Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday
, Matthew N. Van Ert
, Tatum S. Simonson
, David M. Wagner
, Leo J. Kenefic
, Christopher J. Allender
, Paul Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Original language	English (US)
Pages (from-to)	1995-1997
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	4
DOIs	https://doi.org/10.1128/JCM.43.4.1995-1997.2005
State	Published - Apr 2005

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.43.4.1995-1997.2005

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abstract = "A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.",

author = "Easterday, \{W. Ryan\} and \{Van Ert\}, \{Matthew N.\} and Simonson, \{Tatum S.\} and Wagner, \{David M.\} and Kenefic, \{Leo J.\} and Allender, \{Christopher J.\} and Paul Keim",

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AU - Easterday, W. Ryan

AU - Van Ert, Matthew N.

AU - Simonson, Tatum S.

AU - Wagner, David M.

AU - Kenefic, Leo J.

AU - Allender, Christopher J.

AU - Keim, Paul

PY - 2005/4

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AB - A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

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DO - 10.1128/JCM.43.4.1995-1997.2005

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AN - SCOPUS:16844371998

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Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

Abstract

ASJC Scopus subject areas

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