Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday; Matthew N. Van Ert; Tatum S. Simonson; David M. Wagner; Leo J. Kenefic; Christopher J. Allender; Paul Keim

doi:10.1128/JCM.43.4.1995-1997.2005

Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday, Matthew N. Van Ert, Tatum S. Simonson, David M. Wagner, Leo J. Kenefic, Christopher J. Allender, Paul Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Original language	English (US)
Pages (from-to)	1995-1997
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	4
DOIs	https://doi.org/10.1128/JCM.43.4.1995-1997.2005
State	Published - Apr 2005

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.43.4.1995-1997.2005

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abstract = "A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.",

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AU - Simonson, Tatum S.

AU - Wagner, David M.

AU - Kenefic, Leo J.

AU - Allender, Christopher J.

AU - Keim, Paul

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AB - A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

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Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

Abstract

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