Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren; Matthew N. Van Ert; James M. Schupp; W. Ryan Easterday; Tatum S. Simonson; Richard T. Okinaka; Talima Pearson; Paul Keim

doi:10.1128/JCM.43.11.5771-5774.2005

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren
, Matthew N. Van Ert
, James M. Schupp
, W. Ryan Easterday
, Tatum S. Simonson
, Richard T. Okinaka
, Talima Pearson
, Paul Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

Original language	English (US)
Pages (from-to)	5771-5774
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	11
DOIs	https://doi.org/10.1128/JCM.43.11.5771-5774.2005
State	Published - Nov 2005

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.43.11.5771-5774.2005

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title = "Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei",

abstract = "A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.",

author = "U'Ren, \{Jana M.\} and \{Van Ert\}, \{Matthew N.\} and Schupp, \{James M.\} and Easterday, \{W. Ryan\} and Simonson, \{Tatum S.\} and Okinaka, \{Richard T.\} and Talima Pearson and Paul Keim",

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AU - U'Ren, Jana M.

AU - Van Ert, Matthew N.

AU - Schupp, James M.

AU - Easterday, W. Ryan

AU - Simonson, Tatum S.

AU - Okinaka, Richard T.

AU - Pearson, Talima

AU - Keim, Paul

PY - 2005/11

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N2 - A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

AB - A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

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U2 - 10.1128/JCM.43.11.5771-5774.2005

DO - 10.1128/JCM.43.11.5771-5774.2005

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AN - SCOPUS:27744574381

SN - 0095-1137

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JF - Journal of Clinical Microbiology

IS - 11

ER -

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Abstract

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