Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren; Matthew N. Van Ert; James M. Schupp; W. Ryan Easterday; Tatum S. Simonson; Richard T. Okinaka; Talima Pearson; Paul Keim

doi:10.1128/JCM.43.11.5771-5774.2005

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren, Matthew N. Van Ert, James M. Schupp, W. Ryan Easterday, Tatum S. Simonson, Richard T. Okinaka, Talima Pearson, Paul Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

Original language	English (US)
Pages (from-to)	5771-5774
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	11
DOIs	https://doi.org/10.1128/JCM.43.11.5771-5774.2005
State	Published - Nov 2005

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/JCM.43.11.5771-5774.2005

Cite this

@article{d44c2980f00b4722bb476958ef14a6fb,

title = "Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei",

abstract = "A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.",

author = "U'Ren, {Jana M.} and {Van Ert}, {Matthew N.} and Schupp, {James M.} and Easterday, {W. Ryan} and Simonson, {Tatum S.} and Okinaka, {Richard T.} and Talima Pearson and Paul Keim",

year = "2005",

month = nov,

doi = "10.1128/JCM.43.11.5771-5774.2005",

language = "English (US)",

volume = "43",

pages = "5771--5774",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

AU - U'Ren, Jana M.

AU - Van Ert, Matthew N.

AU - Schupp, James M.

AU - Easterday, W. Ryan

AU - Simonson, Tatum S.

AU - Okinaka, Richard T.

AU - Pearson, Talima

AU - Keim, Paul

PY - 2005/11

Y1 - 2005/11

N2 - A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

AB - A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

UR - http://www.scopus.com/inward/record.url?scp=27744574381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744574381&partnerID=8YFLogxK

U2 - 10.1128/JCM.43.11.5771-5774.2005

DO - 10.1128/JCM.43.11.5771-5774.2005

M3 - Article

C2 - 16272516

AN - SCOPUS:27744574381

SN - 0095-1137

VL - 43

SP - 5771

EP - 5774

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this