3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Microbiological Methods|
|State||Published - Dec 2003|
ASJC Scopus subject areas
- Molecular Biology
- Microbiology (medical)