Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Mary E. Kauffman; William K. Keener; Scott R. Clingenpeel; Mary E. Watwood; David W. Reed; Yoshiko Fujita; R. Michael Lehman

doi:10.1016/j.mimet.2003.07.001

Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Mary E. Kauffman, William K. Keener, Scott R. Clingenpeel, Mary E. Watwood, David W. Reed, Yoshiko Fujita, R. Michael Lehman

Biological Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

Original language	English (US)
Pages (from-to)	801-805
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	55
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2003.07.001
State	Published - Dec 2003

Keywords

3-Hydroxyphenylacetylene
Activity
Chromogenic
Fluorogenic
Phenylacetylene
Toluene

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2003.07.001

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title = "Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol",

abstract = "3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.",

keywords = "3-Hydroxyphenylacetylene, Activity, Chromogenic, Fluorogenic, Phenylacetylene, Toluene",

author = "Kauffman, {Mary E.} and Keener, {William K.} and Clingenpeel, {Scott R.} and Watwood, {Mary E.} and Reed, {David W.} and Yoshiko Fujita and Lehman, {R. Michael}",

note = "Funding Information: Funding for this project was provided by the U.S. Department of Energy, Office of Environmental Management at the Idaho National Engineering and Environmental Laboratory (INEEL), operated by Bechtel BWXT Idaho, LLC, under contract DE-AC07-99ID13727.",

year = "2003",

month = dec,

doi = "10.1016/j.mimet.2003.07.001",

language = "English (US)",

volume = "55",

pages = "801--805",

journal = "Journal of Microbiological Methods",

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T1 - Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

AU - Kauffman, Mary E.

AU - Keener, William K.

AU - Clingenpeel, Scott R.

AU - Watwood, Mary E.

AU - Reed, David W.

AU - Fujita, Yoshiko

AU - Lehman, R. Michael

N1 - Funding Information: Funding for this project was provided by the U.S. Department of Energy, Office of Environmental Management at the Idaho National Engineering and Environmental Laboratory (INEEL), operated by Bechtel BWXT Idaho, LLC, under contract DE-AC07-99ID13727.

PY - 2003/12

Y1 - 2003/12

N2 - 3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

AB - 3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

KW - 3-Hydroxyphenylacetylene

KW - Activity

KW - Chromogenic

KW - Fluorogenic

KW - Phenylacetylene

KW - Toluene

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ER -

Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Abstract

Keywords

ASJC Scopus subject areas

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