Ultra-Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks

Ren Liu, Jihwan Lee, Youngbin Tchoe, Deborah Pre, Andrew M. Bourhis, Agnieszka D'Antonio-Chronowska, Gaelle Robin, Sang Heon Lee, Yun Goo Ro, Ritwik Vatsyayan, Karen J. Tonsfeldt, Lorraine A. Hossain, M. Lisa Phipps, Jinkyoung Yoo, John Nogan, Jennifer S. Martinez, Kelly A. Frazer, Anne G. Bang, Shadi A. Dayeh

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Intracellular access with high spatiotemporal resolution can enhance the understanding of how neurons or cardiomyocytes regulate and orchestrate network activity and how this activity can be affected with pharmacology or other interventional modalities. Nanoscale devices often employ electroporation to transiently permeate the cell membrane and record intracellular potentials, which tend to decrease rapidly with time. Here, one reports innovative scalable, vertical, ultrasharp nanowire arrays that are individually addressable to enable long-term, native recordings of intracellular potentials. One reports electrophysiological recordings that are indicative of intracellular access from 3D tissue-like networks of neurons and cardiomyocytes across recording days and that do not decrease to extracellular amplitudes for the duration of the recording of several minutes. The findings are validated with cross-sectional microscopy, pharmacology, and electrical interventions. The experiments and simulations demonstrate that the individual electrical addressability of nanowires is necessary for high-fidelity intracellular electrophysiological recordings. This study advances the understanding of and control over high-quality multichannel intracellular recordings and paves the way toward predictive, high-throughput, and low-cost electrophysiological drug screening platforms.

Original languageEnglish (US)
Article number2108378
JournalAdvanced Functional Materials
Volume32
Issue number8
DOIs
StatePublished - Feb 16 2022

Keywords

  • cardiomyocytes
  • culture
  • intracellular
  • nanowires
  • neurons
  • tissues

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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