@article{15956d80420543149b96c6b6f9821d10,
title = "Ultra-Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks",
abstract = "Intracellular access with high spatiotemporal resolution can enhance the understanding of how neurons or cardiomyocytes regulate and orchestrate network activity and how this activity can be affected with pharmacology or other interventional modalities. Nanoscale devices often employ electroporation to transiently permeate the cell membrane and record intracellular potentials, which tend to decrease rapidly with time. Here, one reports innovative scalable, vertical, ultrasharp nanowire arrays that are individually addressable to enable long-term, native recordings of intracellular potentials. One reports electrophysiological recordings that are indicative of intracellular access from 3D tissue-like networks of neurons and cardiomyocytes across recording days and that do not decrease to extracellular amplitudes for the duration of the recording of several minutes. The findings are validated with cross-sectional microscopy, pharmacology, and electrical interventions. The experiments and simulations demonstrate that the individual electrical addressability of nanowires is necessary for high-fidelity intracellular electrophysiological recordings. This study advances the understanding of and control over high-quality multichannel intracellular recordings and paves the way toward predictive, high-throughput, and low-cost electrophysiological drug screening platforms.",
keywords = "cardiomyocytes, culture, intracellular, nanowires, neurons, tissues",
author = "Ren Liu and Jihwan Lee and Youngbin Tchoe and Deborah Pre and Bourhis, {Andrew M.} and Agnieszka D'Antonio-Chronowska and Gaelle Robin and Lee, {Sang Heon} and Ro, {Yun Goo} and Ritwik Vatsyayan and Tonsfeldt, {Karen J.} and Hossain, {Lorraine A.} and Phipps, {M. Lisa} and Jinkyoung Yoo and John Nogan and Martinez, {Jennifer S.} and Frazer, {Kelly A.} and Bang, {Anne G.} and Dayeh, {Shadi A.}",
note = "Funding Information: R.L., J.L., and Y.T. contributed equally to this work. This work was performed with the gracious support of National Science Foundation Award No. 1728497 under the stewardship of Dr. Khershed Cooper and of the National Institutes of Health Award No. NBIB DP2‐EB029757 under the stewardship of Dr. Michael Wolfson. S.A.D., J.S.M., and R.L. also acknowledge the gracious support of the UC‐National Laboratory in Residence Graduate Fellowships (UC‐NLGF), Award No. 477131. This work was performed, in part, at the Center for Integrated Nanotechnologies, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science. Los Alamos National Laboratory, an affirmative action equal opportunity employer, is managed by Triad National Security, LLC for the U.S. Department of Energy's NNSA, under contract 89233218CNA000001 and Sandia National Laboratories (Contract No. DE‐AC04‐94AL85000) through a CINT user proposal. This work was performed in part at the San Diego Nanotechnology Infrastructure (SDNI) of UCSD, a member of the National Nanotechnology Coordinated Infrastructure, which is supported by the National Science Foundation (Grant ECCS‐1542148). The authors thank technical support from the Integration Laboratory at CINT and from the nano3 clean room facilities at UC San Diego's Qualcomm Institute. R.L. and S.A.D. acknowledge inspiring technical discussions with Dr. Renjie Chen, and Dr. Atsunori Tanaka, as well as discussions and technical support from Dr. Katherine L. Jungjohann, Anthony R. James, Douglas V. Pete, and Denise B. Webb of Sandia National Laboratories. S.A.D. conceived and led all aspects of the project. R.L. developed the fabrication process with S.A.D. R.L. fabricated the platform with input and training from J.N., J.Y., M.L.P. and J.S.M; Y.G.R., Y.T. and L.A.H. participated in the fabrication; R.L. performed the FIB sectioning and SEM imaging. R.L. and R.V. performed electrochemical characterization; R.L. and S.H.L. performed electrophysiological recordings; D.P. and G.R. cultured the primary rodent neurons under supervision of A.G.B.; A.D.C. cultured iPSC‐derived cardiovascular progenitor cells under the supervision of K.A.F.; A.M.B., Y.T. and S.A.D. carried out the electrochemical interface modeling. J.L., Y.T., R.L., K.J.T., and S.A.D. analyzed the data and wrote the manuscript. All authors discussed the results and contributed to the manuscript writing. Funding Information: R.L., J.L., and Y.T. contributed equally to this work. This work was performed with the gracious support of National Science Foundation Award No. 1728497 under the stewardship of Dr. Khershed Cooper and of the National Institutes of Health Award No. NBIB DP2-EB029757 under the stewardship of Dr. Michael Wolfson. S.A.D., J.S.M., and R.L. also acknowledge the gracious support of the UC-National Laboratory in Residence Graduate Fellowships (UC-NLGF), Award No. 477131. This work was performed, in part, at the Center for Integrated Nanotechnologies, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science. Los Alamos National Laboratory, an affirmative action equal opportunity employer, is managed by Triad National Security, LLC for the U.S. Department of Energy's NNSA, under contract 89233218CNA000001 and Sandia National Laboratories (Contract No. DE-AC04-94AL85000) through a CINT user proposal. This work was performed in part at the San Diego Nanotechnology Infrastructure (SDNI) of UCSD, a member of the National Nanotechnology Coordinated Infrastructure, which is supported by the National Science Foundation (Grant ECCS-1542148). The authors thank technical support from the Integration Laboratory at CINT and from the nano3 clean room facilities at UC San Diego's Qualcomm Institute. R.L. and S.A.D. acknowledge inspiring technical discussions with Dr. Renjie Chen, and Dr. Atsunori Tanaka, as well as discussions and technical support from Dr. Katherine L. Jungjohann, Anthony R. James, Douglas V. Pete, and Denise B. Webb of Sandia National Laboratories. S.A.D. conceived and led all aspects of the project. R.L. developed the fabrication process with S.A.D. R.L. fabricated the platform with input and training from J.N., J.Y., M.L.P. and J.S.M; Y.G.R., Y.T. and L.A.H. participated in the fabrication; R.L. performed the FIB sectioning and SEM imaging. R.L. and R.V. performed electrochemical characterization; R.L. and S.H.L. performed electrophysiological recordings; D.P. and G.R. cultured the primary rodent neurons under supervision of A.G.B.; A.D.C. cultured iPSC-derived cardiovascular progenitor cells under the supervision of K.A.F.; A.M.B., Y.T. and S.A.D. carried out the electrochemical interface modeling. J.L., Y.T., R.L., K.J.T., and S.A.D. analyzed the data and wrote the manuscript. All authors discussed the results and contributed to the manuscript writing. Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH.",
year = "2022",
month = feb,
day = "16",
doi = "10.1002/adfm.202108378",
language = "English (US)",
volume = "32",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-VCH Verlag",
number = "8",
}