Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn 10 insertion

Herbert Schweizer; Winfried Boos

doi:10.1007/BF00327683

Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn 10 insertion

Herbert Schweizer, Winfried Boos

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Abstract

To facilitate construction of mutants harboring Δlac for use in gene fusion studies, strains were constructed that carry the trasposon Tn 10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.

Original language	English (US)
Pages (from-to)	293-294
Number of pages	2
Journal	MGG Molecular & General Genetics
Volume	192
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00327683
State	Published - Oct 1983
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn 10 insertion",

abstract = "To facilitate construction of mutants harboring Δlac for use in gene fusion studies, strains were constructed that carry the trasposon Tn 10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.",

author = "Herbert Schweizer and Winfried Boos",

year = "1983",

month = oct,

doi = "10.1007/BF00327683",

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journal = "MGG Molecular & General Genetics",

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T1 - Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn 10 insertion

AU - Schweizer, Herbert

AU - Boos, Winfried

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Y1 - 1983/10

N2 - To facilitate construction of mutants harboring Δlac for use in gene fusion studies, strains were constructed that carry the trasposon Tn 10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.

AB - To facilitate construction of mutants harboring Δlac for use in gene fusion studies, strains were constructed that carry the trasposon Tn 10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.

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JO - MGG Molecular & General Genetics

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Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn 10 insertion

Abstract

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