Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson; Mirjam Kaestli; Mark Mayo; Jolene R. Bowers; Apichai Tuanyok; Jim Schupp; David Engelthaler; David M. Wagner; Paul S. Keim; Bart J. Currie

doi:10.1016/j.mimet.2011.10.023

Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson
, Mirjam Kaestli
, Mark Mayo
, Jolene R. Bowers
, Apichai Tuanyok
, Jim Schupp
, David Engelthaler
, David M. Wagner
, Paul S. Keim
, Bart J. Currie

Biological Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Original language	English (US)
Pages (from-to)	179-181
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2011.10.023
State	Published - Jan 2012

Keywords

Burkholderia pseudomallei
Clinical specimen
DNA extraction
Diagnostic
Melioidosis
Real-time PCR

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2011.10.023

Cite this

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title = "Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens",

abstract = "Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.",

keywords = "Burkholderia pseudomallei, Clinical specimen, DNA extraction, Diagnostic, Melioidosis, Real-time PCR",

author = "Richardson, \{Leisha J.\} and Mirjam Kaestli and Mark Mayo and Bowers, \{Jolene R.\} and Apichai Tuanyok and Jim Schupp and David Engelthaler and Wagner, \{David M.\} and Keim, \{Paul S.\} and Currie, \{Bart J.\}",

note = "Funding Information: We would like to thank the Microbiology Laboratory staff and our medical and nursing colleagues at Royal Darwin Hospital for assistance in patient diagnosis and sample collection, Linda Ward for database support and Eleanor Woolveridge and Alex Humphrey for laboratory assistance. This project was funded by the Australian National Health and Medical Research Council (Project Grant 605820 ) and grants NIH NIAID UO1-A1075568 and NIH NIAID U54-65359 .",

year = "2012",

month = jan,

doi = "10.1016/j.mimet.2011.10.023",

language = "English (US)",

volume = "88",

pages = "179--181",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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T2 - Comparison of DNA extraction methods from clinical specimens

AU - Richardson, Leisha J.

AU - Kaestli, Mirjam

AU - Mayo, Mark

AU - Bowers, Jolene R.

AU - Tuanyok, Apichai

AU - Schupp, Jim

AU - Engelthaler, David

AU - Wagner, David M.

AU - Keim, Paul S.

AU - Currie, Bart J.

N1 - Funding Information: We would like to thank the Microbiology Laboratory staff and our medical and nursing colleagues at Royal Darwin Hospital for assistance in patient diagnosis and sample collection, Linda Ward for database support and Eleanor Woolveridge and Alex Humphrey for laboratory assistance. This project was funded by the Australian National Health and Medical Research Council (Project Grant 605820 ) and grants NIH NIAID UO1-A1075568 and NIH NIAID U54-65359 .

PY - 2012/1

Y1 - 2012/1

N2 - Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

AB - Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

KW - Burkholderia pseudomallei

KW - Clinical specimen

KW - DNA extraction

KW - Diagnostic

KW - Melioidosis

KW - Real-time PCR

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U2 - 10.1016/j.mimet.2011.10.023

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Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Abstract

Keywords

ASJC Scopus subject areas

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