@article{02c88c27b48a41e18ef7170704e7adab,
title = "Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens",
abstract = "Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.",
keywords = "Burkholderia pseudomallei, Clinical specimen, DNA extraction, Diagnostic, Melioidosis, Real-time PCR",
author = "Richardson, {Leisha J.} and Mirjam Kaestli and Mark Mayo and Bowers, {Jolene R.} and Apichai Tuanyok and Jim Schupp and David Engelthaler and Wagner, {David M.} and Keim, {Paul S.} and Currie, {Bart J.}",
note = "Funding Information: We would like to thank the Microbiology Laboratory staff and our medical and nursing colleagues at Royal Darwin Hospital for assistance in patient diagnosis and sample collection, Linda Ward for database support and Eleanor Woolveridge and Alex Humphrey for laboratory assistance. This project was funded by the Australian National Health and Medical Research Council (Project Grant 605820 ) and grants NIH NIAID UO1-A1075568 and NIH NIAID U54-65359 .",
year = "2012",
month = jan,
doi = "10.1016/j.mimet.2011.10.023",
language = "English (US)",
volume = "88",
pages = "179--181",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",
}