@inbook{25878c221ce84601a835087be74c47f3,
title = "Tn7-based single-copy insertion vectors for acinetobacter baumannii",
abstract = "Acinetobacter baumannii is considered a problematic Gram-negative pathogen due to its widespread resistance to antibiotics. Understanding of resistance mechanisms in A. baumannii is critical for designing new and effective therapeutic options. However, this is hampered by the lack of tools to carry out genetic manipulations in A. baumannii. Here, we describe methods to use a chromosomal mini-Tn7-based single-copy gene expression system in A. baumannii. This system can be effectively used for performing genetic complementation studies, for tagging with fluorescent proteins, or for reporter fusion assays.",
keywords = "Electroporation, Four-parental mating, Gene complementation, Gene expression",
author = "Kaleigh Ducas-Mowchun and {De Silva}, {P. Malaka} and Rakesh Patidar and Schweizer, {Herbert P.} and Ayush Kumar",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9118-1_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "135--150",
booktitle = "Methods in Molecular Biology",
}