Tn7-based single-copy insertion vectors for acinetobacter baumannii

Kaleigh Ducas-Mowchun, P. Malaka De Silva, Rakesh Patidar, Herbert P. Schweizer, Ayush Kumar

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


Acinetobacter baumannii is considered a problematic Gram-negative pathogen due to its widespread resistance to antibiotics. Understanding of resistance mechanisms in A. baumannii is critical for designing new and effective therapeutic options. However, this is hampered by the lack of tools to carry out genetic manipulations in A. baumannii. Here, we describe methods to use a chromosomal mini-Tn7-based single-copy gene expression system in A. baumannii. This system can be effectively used for performing genetic complementation studies, for tagging with fluorescent proteins, or for reporter fusion assays.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages16
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Electroporation
  • Four-parental mating
  • Gene complementation
  • Gene expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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