The utility of differential scanning calorimetry curves of blood plasma for diagnosis, subtype differentiation and predicted survival in lung cancer

Gabriela Schneider, Alagammai Kaliappan, Taylor Q. Nguyen, Robert Buscaglia, Guy N. Brock, Melissa Barousse Hall, Crissie Despirito, Daniel W. Wilkey, Michael L. Merchant, Jon B. Klein, Tanya A. Wiese, Hiram L. Rivas-Perez, Goetz H. Kloecker, Nichola C. Garbett

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Early detection of lung cancer (LC) significantly increases the likelihood of successful treatment and improves LC survival rates. Currently, screening (mainly low-dose CT scans) is recommended for individuals at high risk. However, the recent increase in the number of LC cases unrelated to the well-known risk factors, and the high false-positive rate of low-dose CT, indicate a need to develop new, non-invasive methods for LC detection. Therefore, we evaluated the use of differential scanning calorimetry (DSC) for LC patients’ diagnosis and predicted survival. Addition-ally, by applying mass spectrometry, we investigated whether changes in O-and N-glycosylation of plasma proteins could be an underlying mechanism responsible for observed differences in DSC curves of LC and control subjects. Our results indicate selected DSC curve features could be useful for differentiation of LC patients from controls with some capable of distinction between subtypes and stages of LC. DSC curve features also correlate with LC patients’ overall/progression free survival. Moreover, the development of classification models combining patients’ DSC curves with selected plasma protein glycosylation levels that changed in the presence of LC could improve the sensitivity and specificity of the detection of LC. With further optimization and development of the classification method, DSC could provide an accurate, non-invasive, radiation-free strategy for LC screening and diagnosis.

Original languageEnglish (US)
Article number5326
JournalCancers
Volume13
Issue number21
DOIs
StatePublished - Nov 1 2021

Keywords

  • DSC curve
  • Diagnosis
  • Differential scanning calorimetry (DSC)
  • Lung cancer
  • Overall survival
  • Progression-free survival

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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