The skeletal muscle VEGF mRNA response to acute exercise in patients with chronic heart failure

Fabio Esposito; Odile Mathieu-Costello; Pauline L. Entin; Peter D. Wagner; Russell S. Richardson

doi:10.3109/08977190903512602

The skeletal muscle VEGF mRNA response to acute exercise in patients with chronic heart failure

Fabio Esposito, Odile Mathieu-Costello, Pauline L. Entin, Peter D. Wagner, Russell S. Richardson

Biological Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

In seven patients with chronic heart failure (CHF) and six controls, we examined (a) resting and post-exercise muscle vascular endothelial growth factor (VEGF) mRNA levels and (b) their relationship with muscle structure and function. Muscle biopsies were taken after 30 min of single-leg knee-extensor exercise at 50% of maximum work rate (50% WR_max) from both the exercised and rested legs. Muscle blood flow(Q) and O₂ uptake VO ₂were measured during exercise. Resting VEGF mRNA levels were not different between patients and controls and both groups upregulated VEGF mRNA equally in response to acute exercise. Patients had lower Q, VO₂, and mitochondrial density but similar capillarity and fiber area. These findings reveal a normal basal level of muscle VEGF mRNA, its appropriate upregulation in response to acute exercise and, despite increased vascular resistance during exercise, a normal skeletal muscle vascular structure in patients with CHF.

Original language	English (US)
Pages (from-to)	139-147
Number of pages	9
Journal	Growth Factors
Volume	28
Issue number	2
DOIs	https://doi.org/10.3109/08977190903512602
State	Published - Apr 2010

Keywords

Acute exercise
CHF
Morphology
Muscle
VEGF

ASJC Scopus subject areas

Endocrinology
Clinical Biochemistry
Cell Biology

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10.3109/08977190903512602

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title = "The skeletal muscle VEGF mRNA response to acute exercise in patients with chronic heart failure",

abstract = "In seven patients with chronic heart failure (CHF) and six controls, we examined (a) resting and post-exercise muscle vascular endothelial growth factor (VEGF) mRNA levels and (b) their relationship with muscle structure and function. Muscle biopsies were taken after 30 min of single-leg knee-extensor exercise at 50% of maximum work rate (50% WRmax) from both the exercised and rested legs. Muscle blood flow(Q) and O2 uptake VO 2were measured during exercise. Resting VEGF mRNA levels were not different between patients and controls and both groups upregulated VEGF mRNA equally in response to acute exercise. Patients had lower Q, VO2, and mitochondrial density but similar capillarity and fiber area. These findings reveal a normal basal level of muscle VEGF mRNA, its appropriate upregulation in response to acute exercise and, despite increased vascular resistance during exercise, a normal skeletal muscle vascular structure in patients with CHF.",

keywords = "Acute exercise, CHF, Morphology, Muscle, VEGF",

author = "Fabio Esposito and Odile Mathieu-Costello and Entin, {Pauline L.} and Wagner, {Peter D.} and Richardson, {Russell S.}",

note = "Funding Information: The authors thank the patients and control subjects for their committed participation in this study. At the time of the study, Prof. Esposito was on leave from the University of Brescia, Italy. He was partly supported by {\textquoteleft}Centro per lo Studio del Trattamento dello Scompenso Cardiaco{\textquoteright}, University of Brescia. Funding Information: This work was supported by the National Institutes of Health grants from National Heart, Lung, and Blood Institute (grant no. HL-17731), the Sam and Rose Stein Institute for Research on Aging, and Tobacco-Related Disease Research Program (grant no. 15RT-0100).",

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AU - Richardson, Russell S.

N1 - Funding Information: The authors thank the patients and control subjects for their committed participation in this study. At the time of the study, Prof. Esposito was on leave from the University of Brescia, Italy. He was partly supported by ‘Centro per lo Studio del Trattamento dello Scompenso Cardiaco’, University of Brescia. Funding Information: This work was supported by the National Institutes of Health grants from National Heart, Lung, and Blood Institute (grant no. HL-17731), the Sam and Rose Stein Institute for Research on Aging, and Tobacco-Related Disease Research Program (grant no. 15RT-0100).

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N2 - In seven patients with chronic heart failure (CHF) and six controls, we examined (a) resting and post-exercise muscle vascular endothelial growth factor (VEGF) mRNA levels and (b) their relationship with muscle structure and function. Muscle biopsies were taken after 30 min of single-leg knee-extensor exercise at 50% of maximum work rate (50% WRmax) from both the exercised and rested legs. Muscle blood flow(Q) and O2 uptake VO 2were measured during exercise. Resting VEGF mRNA levels were not different between patients and controls and both groups upregulated VEGF mRNA equally in response to acute exercise. Patients had lower Q, VO2, and mitochondrial density but similar capillarity and fiber area. These findings reveal a normal basal level of muscle VEGF mRNA, its appropriate upregulation in response to acute exercise and, despite increased vascular resistance during exercise, a normal skeletal muscle vascular structure in patients with CHF.

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The skeletal muscle VEGF mRNA response to acute exercise in patients with chronic heart failure

Abstract

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ASJC Scopus subject areas

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