The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

Fiona S.L. Brinkman, Geoffrey L. Winsor, Rachel E. Done, Alain Filloux, Vanessa I. Francis, Joanna B. Goldberg, E. Peter Greenberg, Kook Han, Robert E.W. Hancock, Cara H. Haney, Susanne Häußler, Jens Klockgether, Iain L. Lamont, Roger C. Levesque, Stephen Lory, Pablo I. Nikel, Steven L. Porter, Matthew W. Scurlock, Herbert P. Schweizer, Burkhard TümmlerMeng Wang, Martin Welch

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time—including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.

Original languageEnglish (US)
Title of host publicationAdvances in Microbial Physiology
EditorsRobert K. Poole
PublisherAcademic Press
Pages25-88
Number of pages64
ISBN (Print)9780128246023
DOIs
StatePublished - Jan 2021

Publication series

NameAdvances in Microbial Physiology
Volume79
ISSN (Print)0065-2911

Keywords

  • Cystic fibrosis
  • PAO1
  • Pseudomonas aeruginosa
  • Whole genome sequence

ASJC Scopus subject areas

  • Microbiology
  • Physiology

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