The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

Fiona S.L. Brinkman; Geoffrey L. Winsor; Rachel E. Done; Alain Filloux; Vanessa I. Francis; Joanna B. Goldberg; E. Peter Greenberg; Kook Han; Robert E.W. Hancock; Cara H. Haney; Susanne Häußler; Jens Klockgether; Iain L. Lamont; Roger C. Levesque; Stephen Lory; Pablo I. Nikel; Steven L. Porter; Matthew W. Scurlock; Herbert P. Schweizer; Burkhard Tümmler; Meng Wang; Martin Welch

doi:10.1016/bs.ampbs.2021.07.001

The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

Fiona S.L. Brinkman, Geoffrey L. Winsor, Rachel E. Done, Alain Filloux, Vanessa I. Francis, Joanna B. Goldberg, E. Peter Greenberg, Kook Han, Robert E.W. Hancock, Cara H. Haney, Susanne Häußler, Jens Klockgether, Iain L. Lamont, Roger C. Levesque, Stephen Lory, Pablo I. Nikel, Steven L. Porter, Matthew W. Scurlock, Herbert P. Schweizer, Burkhard TümmlerMeng Wang, Martin Welch

Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Scopus citations

Abstract

Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time—including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.

Original language	English (US)
Title of host publication	Advances in Microbial Physiology
Editors	Robert K. Poole
Publisher	Academic Press
Pages	25-88
Number of pages	64
ISBN (Print)	9780128246023
DOIs	https://doi.org/10.1016/bs.ampbs.2021.07.001
State	Published - Jan 2021

Publication series

Name	Advances in Microbial Physiology
Volume	79
ISSN (Print)	0065-2911

Keywords

Cystic fibrosis
PAO1
Pseudomonas aeruginosa
Whole genome sequence

ASJC Scopus subject areas

Microbiology
Physiology

Access to Document

10.1016/bs.ampbs.2021.07.001

Cite this

Brinkman, F. S. L., Winsor, G. L., Done, R. E., Filloux, A., Francis, V. I., Goldberg, J. B., Greenberg, E. P., Han, K., Hancock, R. E. W., Haney, C. H., Häußler, S., Klockgether, J., Lamont, I. L., Levesque, R. C., Lory, S., Nikel, P. I., Porter, S. L., Scurlock, M. W., Schweizer, H. P., ... Welch, M. (2021). The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration. In R. K. Poole (Ed.), Advances in Microbial Physiology (pp. 25-88). (Advances in Microbial Physiology; Vol. 79). Academic Press. https://doi.org/10.1016/bs.ampbs.2021.07.001

Brinkman, FSL, Winsor, GL, Done, RE, Filloux, A, Francis, VI, Goldberg, JB, Greenberg, EP, Han, K, Hancock, REW, Haney, CH, Häußler, S, Klockgether, J, Lamont, IL, Levesque, RC, Lory, S, Nikel, PI, Porter, SL, Scurlock, MW, Schweizer, HP, Tümmler, B, Wang, M & Welch, M 2021, The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration. in RK Poole (ed.), Advances in Microbial Physiology. Advances in Microbial Physiology, vol. 79, Academic Press, pp. 25-88. https://doi.org/10.1016/bs.ampbs.2021.07.001

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abstract = "Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time—including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.",

keywords = "Cystic fibrosis, PAO1, Pseudomonas aeruginosa, Whole genome sequence",

author = "Brinkman, {Fiona S.L.} and Winsor, {Geoffrey L.} and Done, {Rachel E.} and Alain Filloux and Francis, {Vanessa I.} and Goldberg, {Joanna B.} and Greenberg, {E. Peter} and Kook Han and Hancock, {Robert E.W.} and Haney, {Cara H.} and Susanne H{\"a}u{\ss}ler and Jens Klockgether and Lamont, {Iain L.} and Levesque, {Roger C.} and Stephen Lory and Nikel, {Pablo I.} and Porter, {Steven L.} and Scurlock, {Matthew W.} and Schweizer, {Herbert P.} and Burkhard T{\"u}mmler and Meng Wang and Martin Welch",

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year = "2021",

month = jan,

doi = "10.1016/bs.ampbs.2021.07.001",

language = "English (US)",

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AU - Nikel, Pablo I.

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ER -

The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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