The developmental expression of cysteine proteinases in Schistosoma mansoni

Fernando P. Monroy; Marc H. Dresden

doi:10.1016/0020-7519(95)00077-1

The developmental expression of cysteine proteinases in Schistosoma mansoni

Fernando P. Monroy, Marc H. Dresden

Biological Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In this study several probes were used to identify and characterize cysteine proteinases (CP) from Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[¹²⁵I]tyr-ala-CHN₂. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in S. mansoni.

Original language	English (US)
Pages (from-to)	109-112
Number of pages	4
Journal	International Journal for Parasitology
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/0020-7519(95)00077-1
State	Published - Jan 1996

Keywords

Cathepsins
Cysteine proteinases
Enzyme inhibitors
Proteases
Proteinases
S. mansoni
Schistosomes
Trematode

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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10.1016/0020-7519(95)00077-1

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title = "The developmental expression of cysteine proteinases in Schistosoma mansoni",

abstract = "In this study several probes were used to identify and characterize cysteine proteinases (CP) from Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[125I]tyr-ala-CHN2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in S. mansoni.",

keywords = "Cathepsins, Cysteine proteinases, Enzyme inhibitors, Proteases, Proteinases, S. mansoni, Schistosomes, Trematode",

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AU - Monroy, Fernando P.

AU - Dresden, Marc H.

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AB - In this study several probes were used to identify and characterize cysteine proteinases (CP) from Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[125I]tyr-ala-CHN2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in S. mansoni.

KW - Cathepsins

KW - Cysteine proteinases

KW - Enzyme inhibitors

KW - Proteases

KW - Proteinases

KW - S. mansoni

KW - Schistosomes

KW - Trematode

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The developmental expression of cysteine proteinases in Schistosoma mansoni

Abstract

Keywords

ASJC Scopus subject areas

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