Temperature-dependent morphology of hybrid nanoflowers from elastin-like polypeptides

Koushik Ghosh, Eva Rose M. Balog, Prakash Sista, Darrick J. Williams, Daniel Kelly, Jennifer S. Martinez, Reginaldo C. Rocha

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


We report a method for creating hybrid organic-inorganic "nanoflowers" using calcium or copper ions as the inorganic component and a recombinantly expressed elastin-like polypeptide (ELP) as the organic component. Polypeptides provide binding sites for the dynamic coordination with metal ions, and then such noncovalent complexes become nucleation sites for primary crystals of metal phosphates. We have shown that the interaction between the stimuli-responsive ELP and Ca2+ or Cu2+, in the presence of phosphate, leads to the growth of micrometer-sized particles featuring nanoscale patterns shaped like flower petals. The morphology of these flower-like composite structures is dependent upon the temperature of growth and has been characterized by scanning electron microscopy. The composition of nanoflowers has also been analyzed by energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The temperature-dependent morphologies of these hybrid nanostructures, which arise from the controllable phase transition of ELPs, hold potential for morphological control of biomaterials in emerging applications such as tissue engineering and biocatalysis.

Original languageEnglish (US)
Article number021101
JournalAPL Materials
Issue number2
StatePublished - Feb 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering


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