Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147

Joshua K. Stone; Christian Heiss; Zhirui Wang; Ian Black; Stephanie A. Grasso; Andrew T. Koppisch; Parastoo Azadi; Paul Keim; Apichai Tuanyok

doi:10.1016/j.carres.2013.08.011

Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147

Joshua K. Stone, Christian Heiss, Zhirui Wang, Ian Black, Stephanie A. Grasso, Andrew T. Koppisch, Parastoo Azadi, Paul Keim, Apichai Tuanyok

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D ¹H and ¹³C NMR spectroscopy showed that the O-antigen was a repeating disaccharide with the following structure: [3)-β-d-Glcp-(1→3)-2OAc-α-l-6dTalp-(1→] _n NMR spectroscopy also revealed the presence of a co-extracted exopolysaccharide previously described in B. pseudomallei, with the structure: [3)-2OAc-β-d-Galp-(1→4)-α-d-Galp-(1→3)-β-d-Galp- (1→5)-β-d-Kdop-(2→]_n.

Original language	English (US)
Pages (from-to)	68-72
Number of pages	5
Journal	Carbohydrate Research
Volume	386
Issue number	1
DOIs	https://doi.org/10.1016/j.carres.2013.08.011
State	Published - Mar 11 2014

Keywords

Burkholderia oklahomensis
Exopolysaccharide
Lipopolysaccharide
NMR
O-antigen
Structure

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.carres.2013.08.011

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title = "Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147",

abstract = "Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D 1H and 13C NMR spectroscopy showed that the O-antigen was a repeating disaccharide with the following structure: [3)-β-d-Glcp-(1→3)-2OAc-α-l-6dTalp-(1→] n NMR spectroscopy also revealed the presence of a co-extracted exopolysaccharide previously described in B. pseudomallei, with the structure: [3)-2OAc-β-d-Galp-(1→4)-α-d-Galp-(1→3)-β-d-Galp- (1→5)-β-d-Kdop-(2→]n.",

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doi = "10.1016/j.carres.2013.08.011",

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AU - Stone, Joshua K.

AU - Heiss, Christian

AU - Wang, Zhirui

AU - Black, Ian

AU - Grasso, Stephanie A.

AU - Koppisch, Andrew T.

AU - Azadi, Parastoo

AU - Keim, Paul

AU - Tuanyok, Apichai

PY - 2014/3/11

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N2 - Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D 1H and 13C NMR spectroscopy showed that the O-antigen was a repeating disaccharide with the following structure: [3)-β-d-Glcp-(1→3)-2OAc-α-l-6dTalp-(1→] n NMR spectroscopy also revealed the presence of a co-extracted exopolysaccharide previously described in B. pseudomallei, with the structure: [3)-2OAc-β-d-Galp-(1→4)-α-d-Galp-(1→3)-β-d-Galp- (1→5)-β-d-Kdop-(2→]n.

AB - Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D 1H and 13C NMR spectroscopy showed that the O-antigen was a repeating disaccharide with the following structure: [3)-β-d-Glcp-(1→3)-2OAc-α-l-6dTalp-(1→] n NMR spectroscopy also revealed the presence of a co-extracted exopolysaccharide previously described in B. pseudomallei, with the structure: [3)-2OAc-β-d-Galp-(1→4)-α-d-Galp-(1→3)-β-d-Galp- (1→5)-β-d-Kdop-(2→]n.

KW - Burkholderia oklahomensis

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KW - Lipopolysaccharide

KW - NMR

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KW - Structure

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Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147

Abstract

Keywords

ASJC Scopus subject areas

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