Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides).

J. H. Adams, I. J. East, F. Monroy, E. A. Washington, C. Dobson

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7 Scopus citations


Antigens were identified from Nematospiroides dubius recovered from outbred Quackenbush mice between 4 and 10 days postinoculation (PI). Parasite surface proteins were radioiodinated and extracts of the whole worms were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and reacted with normal and immune mouse sera followed by an avidin-biotin-peroxidase assay. Antigens ranged between 250,000 and 20,000 molecular weight (MW). A major surface antigen, 60,000 MW, which appeared to be a complex of different antigens, and a 250,000-MW internal antigen were found on fourth-stage (L4) and fifth-stage (L5) larvae 5-10 days PI but not earlier. A group of minor surface antigens (24,000-30,000 MW) were also expressed as larvae molted from L4 to L5, 6 and 7 days PI, but they differed from antigens of similar MW expressed by adult worms. An antigen, 45,000 MW, was detected in worms 5-10 days PI, but it was only expressed on the surface of L5 worms 9 and 10 days PI. We suggest that the antigen(s) common to adults and larvae may account for protective immunity.

Original languageEnglish (US)
Pages (from-to)1164-1168
Number of pages5
JournalThe Journal of parasitology
Issue number6
StatePublished - Dec 1987

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics


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