@inbook{a8b5488d12184b8c85caf505c5db37e6,
title = "Stable Isotope Probing of Microorganisms in Environmental Samples with H218O",
abstract = "We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with H218O. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled with 18O. By measuring how much the DNA of each taxon becomes enriched with 18O when an environmental sample is incubated with H218O, it is feasible to quantify that population{\textquoteright}s DNA replication rate, which is a proxy for growth.",
keywords = "HO, Microbial growth, Stable isotope probing",
author = "Egbert Schwartz and Michaela Hayer and Hungate, {Bruce A.} and Mau, {Rebecca L.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9721-3_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "129--136",
booktitle = "Methods in Molecular Biology",
}