Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing

Faith M. Walker; Jeffrey T. Foster; Kevin P. Drees; Carol L. Chambers

doi:10.1007/s12686-013-0125-0

Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing

Faith M. Walker, Jeffrey T. Foster, Kevin P. Drees, Carol L. Chambers

Research output: Contribution to journal › Letter › peer-review

2 Scopus citations

Abstract

The spotted bat (Euderma maculatum) is a rarely-encountered species for which behavior and population attributes are largely unknown. Using next-generation sequencing, we identified and characterized 17 microsatellite loci, which were screened for 31 individuals from northern Arizona. Allelic diversity, observed heterozygosity, and power of discrimination were high (N_A: 5-8 alleles per locus; H_O: 0.55-0.90; P_ID: 1.2 × 10^-15). All loci were in HWE, there was no evidence of null alleles or linkage disequilibrium, and five loci amplified and were variable in another Vespertillionid (Eptesicus fuscus). We will use these loci to evaluate gene flow and genetic diversity across the range of the spotted bat and determine population size in northern Arizona. The latter information is important to resource managers, who attempt to set mortality thresholds for bats at wind energy facilities in this region.

Original language	English (US)
Pages (from-to)	457-459
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	2
DOIs	https://doi.org/10.1007/s12686-013-0125-0
State	Published - Jun 2014

Keywords

Euderma maculatum
Microsatellite
Next-generation sequencing
Spotted bat

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-013-0125-0

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title = "Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing",

abstract = "The spotted bat (Euderma maculatum) is a rarely-encountered species for which behavior and population attributes are largely unknown. Using next-generation sequencing, we identified and characterized 17 microsatellite loci, which were screened for 31 individuals from northern Arizona. Allelic diversity, observed heterozygosity, and power of discrimination were high (NA: 5-8 alleles per locus; HO: 0.55-0.90; PID: 1.2 × 10-15). All loci were in HWE, there was no evidence of null alleles or linkage disequilibrium, and five loci amplified and were variable in another Vespertillionid (Eptesicus fuscus). We will use these loci to evaluate gene flow and genetic diversity across the range of the spotted bat and determine population size in northern Arizona. The latter information is important to resource managers, who attempt to set mortality thresholds for bats at wind energy facilities in this region.",

keywords = "Euderma maculatum, Microsatellite, Next-generation sequencing, Spotted bat",

author = "Walker, {Faith M.} and Foster, {Jeffrey T.} and Drees, {Kevin P.} and Chambers, {Carol L.}",

note = "Funding Information: Acknowledgments Funding was provided by the State of Arizona Technology and Research Initiative Fund and the National Park Service. Research was facilitated by the Bureau of Land Management, Kaibab National Forest, and Navajo Nation. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "2014",

month = jun,

doi = "10.1007/s12686-013-0125-0",

language = "English (US)",

volume = "6",

pages = "457--459",

journal = "Conservation Genetics Resources",

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T1 - Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing

AU - Walker, Faith M.

AU - Foster, Jeffrey T.

AU - Drees, Kevin P.

AU - Chambers, Carol L.

N1 - Funding Information: Acknowledgments Funding was provided by the State of Arizona Technology and Research Initiative Fund and the National Park Service. Research was facilitated by the Bureau of Land Management, Kaibab National Forest, and Navajo Nation. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 2014/6

Y1 - 2014/6

N2 - The spotted bat (Euderma maculatum) is a rarely-encountered species for which behavior and population attributes are largely unknown. Using next-generation sequencing, we identified and characterized 17 microsatellite loci, which were screened for 31 individuals from northern Arizona. Allelic diversity, observed heterozygosity, and power of discrimination were high (NA: 5-8 alleles per locus; HO: 0.55-0.90; PID: 1.2 × 10-15). All loci were in HWE, there was no evidence of null alleles or linkage disequilibrium, and five loci amplified and were variable in another Vespertillionid (Eptesicus fuscus). We will use these loci to evaluate gene flow and genetic diversity across the range of the spotted bat and determine population size in northern Arizona. The latter information is important to resource managers, who attempt to set mortality thresholds for bats at wind energy facilities in this region.

AB - The spotted bat (Euderma maculatum) is a rarely-encountered species for which behavior and population attributes are largely unknown. Using next-generation sequencing, we identified and characterized 17 microsatellite loci, which were screened for 31 individuals from northern Arizona. Allelic diversity, observed heterozygosity, and power of discrimination were high (NA: 5-8 alleles per locus; HO: 0.55-0.90; PID: 1.2 × 10-15). All loci were in HWE, there was no evidence of null alleles or linkage disequilibrium, and five loci amplified and were variable in another Vespertillionid (Eptesicus fuscus). We will use these loci to evaluate gene flow and genetic diversity across the range of the spotted bat and determine population size in northern Arizona. The latter information is important to resource managers, who attempt to set mortality thresholds for bats at wind energy facilities in this region.

KW - Euderma maculatum

KW - Microsatellite

KW - Next-generation sequencing

KW - Spotted bat

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JO - Conservation Genetics Resources

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IS - 2

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Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing

Abstract

Keywords

ASJC Scopus subject areas

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