A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.
|Original language||English (US)|
|Number of pages||3|
|State||Published - May 2010|
ASJC Scopus subject areas
- Molecular Biology