TY - JOUR
T1 - SERS-active Au/SiO2 clouds in powder for rapid ex vivo breast adenocarcinoma diagnosis
AU - Cepeda-Pérez, Elisa
AU - López-Luke, Tzarara
AU - Salas, Pedro
AU - Plascencia-Villa, Germán
AU - Ponce, Arturo
AU - Vivero-Escoto, Juan
AU - José-Yacamán, Miguel
AU - de La Rosa, Elder
N1 - Publisher Copyright:
© 2016 Optical Society of America.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - In the present work, we report a dry-based application technique of Au/SiO2 clouds in powder for rapid ex vivo adenocarcinoma diagnosis through surface-enhanced Raman scattering (SERS); using low laser power and an integration time of one second. Several characteristic Raman peaks frequently used for the diagnosis of breast adenocarcinoma in the range of the amide III are successfully enhanced by breading the tissue with Au/SiO2 powder. The SERS activity of these Au/SiO2 powders is attributed to their rapid rehydration upon contact with the wet tissues, which promotes the formation of gold nanoparticle aggregates. The propensity of the Au/SiO2 cloud structures to adsorb biomolecules in the vicinity of the gold nanoparticle clusters promotes the necessary conditions for SERS detection. In addition, electron microscopy, together with elemental analysis, have been used to confirm the structure of the new Au/SiO2 cloud material and to investigate its distribution in breast tissues.
AB - In the present work, we report a dry-based application technique of Au/SiO2 clouds in powder for rapid ex vivo adenocarcinoma diagnosis through surface-enhanced Raman scattering (SERS); using low laser power and an integration time of one second. Several characteristic Raman peaks frequently used for the diagnosis of breast adenocarcinoma in the range of the amide III are successfully enhanced by breading the tissue with Au/SiO2 powder. The SERS activity of these Au/SiO2 powders is attributed to their rapid rehydration upon contact with the wet tissues, which promotes the formation of gold nanoparticle aggregates. The propensity of the Au/SiO2 cloud structures to adsorb biomolecules in the vicinity of the gold nanoparticle clusters promotes the necessary conditions for SERS detection. In addition, electron microscopy, together with elemental analysis, have been used to confirm the structure of the new Au/SiO2 cloud material and to investigate its distribution in breast tissues.
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U2 - 10.1364/BOE.7.002407
DO - 10.1364/BOE.7.002407
M3 - Article
AN - SCOPUS:84971422162
SN - 2156-7085
VL - 7
SP - 2407
EP - 2418
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 6
ER -