Seroepidemiology of Burkholderia pseudomallei, etiologic agent of melioidosis, in the ouest and sud-est departments of Haiti

Thomas A. Weppelmann, Michael H. Norris, Michael E. Von Fricken, Md Siddiqur Rahman Khan, Bernard A. Okech, Anthony P. Cannella, Herbert P. Schweizer, Daniel C. Sanford, Apichai Tuanyok

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Burkholderia pseudomallei, the etiological agent of melioidosis, has been hypothesized to be endemic throughout the Caribbean, including the impoverished nation of Haiti. However, because of the protean clinical manifestations, presence of asymptomatic infections, and limited medical diagnostic capacity, the identification of active melioidosis cases remains challenging. A seroepidemiological study was conducted using a novel enzyme-linked immunosorbent assay (ELISA) to detect antibodies toward B. pseudomallei in the native population. The performance of an indirect ELISA with purified lipopolysaccharide (LPS) from B. pseudomallei was evaluated using serum collected from rhesus macaques exposed to aerosolized B. pseudomallei. After optimization, serum collected from asymptomatic population members (n = 756) was screened for polyvalent (immunoglobulin M [IgM]/immunoglobulin G [IgG]/immunoglobulinA) and monoclonal (IgGorIgM) immunoglobulins against B. pseudomallei LPS. The population seroprevalence was 11.5% (95% confidence interval [CI]: 9.2, 13.8) for polyvalent immunoglobulins, 9.8% (95% CI: 7.7, 11.9) for IgG, and 1.7% (95% CI: 0.8, 2.6%) for IgM. The seroprevalence was not significantly different by gender (P = 0.16), but increased significantly (P < 0.001) with age, yielding an estimated annual seroconversion rate of 1.05% (95% CI: 0.81, 1.3). The detection of both recent (IgM+) and previous (IgG+) exposure to B. pseudomallei provides serological evidence that melioidosis is endemic in Haiti.

Original languageEnglish (US)
Pages (from-to)1222-1228
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number5
StatePublished - 2018
Externally publishedYes

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Parasitology


Dive into the research topics of 'Seroepidemiology of Burkholderia pseudomallei, etiologic agent of melioidosis, in the ouest and sud-est departments of Haiti'. Together they form a unique fingerprint.

Cite this