Selective isolation of Yersinia pestis from plague-infected fleas

Derek S. Sarovich; Rebecca E. Colman; Erin P. Price; Wai Kwan Chung; Judy Lee; James M. Schupp; Kacy R. Cobble; Joseph D. Busch; James Alexander; Paul Keim; David M. Wagner

doi:10.1016/j.mimet.2010.03.019

Selective isolation of Yersinia pestis from plague-infected fleas

Derek S. Sarovich, Rebecca E. Colman, Erin P. Price, Wai Kwan Chung, Judy Lee, James M. Schupp, Kacy R. Cobble, Joseph D. Busch, James Alexander, Paul Keim, David M. Wagner

Biological Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48. h of growth using less selective media.

Original language	English (US)
Pages (from-to)	95-97
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2010.03.019
State	Published - Jul 2010

Keywords

CIN agar
Isolation
Plague
Selective
Yersinia pestis

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

Access to Document

10.1016/j.mimet.2010.03.019

Cite this

@article{328ad4ec379648b4823b1bd83dd8e44b,

title = "Selective isolation of Yersinia pestis from plague-infected fleas",

abstract = "We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48. h of growth using less selective media.",

keywords = "CIN agar, Isolation, Plague, Selective, Yersinia pestis",

author = "Sarovich, {Derek S.} and Colman, {Rebecca E.} and Price, {Erin P.} and Chung, {Wai Kwan} and Judy Lee and Schupp, {James M.} and Cobble, {Kacy R.} and Busch, {Joseph D.} and James Alexander and Paul Keim and Wagner, {David M.}",

note = "Funding Information: This work was supported by NIH-NIAID ( AI070183 ), the Pacific-Southwest Regional Center of Excellence ( AI065359 ), and the Cowden Endowment at Northern Arizona University .",

year = "2010",

month = jul,

doi = "10.1016/j.mimet.2010.03.019",

language = "English (US)",

volume = "82",

pages = "95--97",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Selective isolation of Yersinia pestis from plague-infected fleas

AU - Sarovich, Derek S.

AU - Colman, Rebecca E.

AU - Price, Erin P.

AU - Chung, Wai Kwan

AU - Lee, Judy

AU - Schupp, James M.

AU - Cobble, Kacy R.

AU - Busch, Joseph D.

AU - Alexander, James

AU - Keim, Paul

AU - Wagner, David M.

N1 - Funding Information: This work was supported by NIH-NIAID ( AI070183 ), the Pacific-Southwest Regional Center of Excellence ( AI065359 ), and the Cowden Endowment at Northern Arizona University .

PY - 2010/7

Y1 - 2010/7

N2 - We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48. h of growth using less selective media.

AB - We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48. h of growth using less selective media.

KW - CIN agar

KW - Isolation

KW - Plague

KW - Selective

KW - Yersinia pestis

UR - http://www.scopus.com/inward/record.url?scp=77953542682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953542682&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2010.03.019

DO - 10.1016/j.mimet.2010.03.019

M3 - Article

C2 - 20385178

AN - SCOPUS:77953542682

SN - 0167-7012

VL - 82

SP - 95

EP - 97

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Selective isolation of Yersinia pestis from plague-infected fleas

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this