Selection and characterization of scFv antibodies against the Sin Nombre hantavirus nucleocapsid protein

Nileena Velappan, Jennifer S. Martinez, Rosa Valero, Leslie Chasteen, Liana Ponce, Virginie Bondu-Hawkins, Craig Kelly, Peter Pavlik, Brian Hjelle, Andrew R.M. Bradbury

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in the old world and hantavirus cardio-pulmonary syndrome (HCPS) in the new. Most cases of HCPS in North America are caused by Sin Nombre Virus (SNV). Current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. Detection of viral antigen may facilitate earlier detection of the pathogen. We describe here the characterization of two single-chain Fv antibodies (scFvs), selected from a large naïve phage antibody library, which are capable of identifying the Sin Nombre Virus nucleocapsid protein (SNV-N), with no cross reactivity with the nucleocapsid protein from other hantaviruses. The utility of such selected scFvs was increased by the creation of an scFv-alkaline phosphatase fusion protein which was able to directly detect virally produced material without the need for additional reagents.

Original languageEnglish (US)
Pages (from-to)60-69
Number of pages10
JournalJournal of Immunological Methods
Volume321
Issue number1-2
DOIs
StatePublished - Apr 10 2007
Externally publishedYes

Keywords

  • Hantavirus
  • Nucleocapsid protein
  • Phage antibody
  • Phage display
  • scFv
  • Sin Nombre Virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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