TY - JOUR
T1 - Selection and characterization of scFv antibodies against the Sin Nombre hantavirus nucleocapsid protein
AU - Velappan, Nileena
AU - Martinez, Jennifer S.
AU - Valero, Rosa
AU - Chasteen, Leslie
AU - Ponce, Liana
AU - Bondu-Hawkins, Virginie
AU - Kelly, Craig
AU - Pavlik, Peter
AU - Hjelle, Brian
AU - Bradbury, Andrew R.M.
N1 - Funding Information:
This research was funded by the LDRD-DR program at Los Alamos National Laboratory. We would like to thank Ms. Priya Dighe for her technical assistance.
PY - 2007/4/10
Y1 - 2007/4/10
N2 - Rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in the old world and hantavirus cardio-pulmonary syndrome (HCPS) in the new. Most cases of HCPS in North America are caused by Sin Nombre Virus (SNV). Current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. Detection of viral antigen may facilitate earlier detection of the pathogen. We describe here the characterization of two single-chain Fv antibodies (scFvs), selected from a large naïve phage antibody library, which are capable of identifying the Sin Nombre Virus nucleocapsid protein (SNV-N), with no cross reactivity with the nucleocapsid protein from other hantaviruses. The utility of such selected scFvs was increased by the creation of an scFv-alkaline phosphatase fusion protein which was able to directly detect virally produced material without the need for additional reagents.
AB - Rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in the old world and hantavirus cardio-pulmonary syndrome (HCPS) in the new. Most cases of HCPS in North America are caused by Sin Nombre Virus (SNV). Current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. Detection of viral antigen may facilitate earlier detection of the pathogen. We describe here the characterization of two single-chain Fv antibodies (scFvs), selected from a large naïve phage antibody library, which are capable of identifying the Sin Nombre Virus nucleocapsid protein (SNV-N), with no cross reactivity with the nucleocapsid protein from other hantaviruses. The utility of such selected scFvs was increased by the creation of an scFv-alkaline phosphatase fusion protein which was able to directly detect virally produced material without the need for additional reagents.
KW - Hantavirus
KW - Nucleocapsid protein
KW - Phage antibody
KW - Phage display
KW - Sin Nombre Virus
KW - scFv
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U2 - 10.1016/j.jim.2007.01.011
DO - 10.1016/j.jim.2007.01.011
M3 - Article
C2 - 17336997
AN - SCOPUS:33947539526
SN - 0022-1759
VL - 321
SP - 60
EP - 69
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -