Abstract
Rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in the old world and hantavirus cardio-pulmonary syndrome (HCPS) in the new. Most cases of HCPS in North America are caused by Sin Nombre Virus (SNV). Current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. Detection of viral antigen may facilitate earlier detection of the pathogen. We describe here the characterization of two single-chain Fv antibodies (scFvs), selected from a large naïve phage antibody library, which are capable of identifying the Sin Nombre Virus nucleocapsid protein (SNV-N), with no cross reactivity with the nucleocapsid protein from other hantaviruses. The utility of such selected scFvs was increased by the creation of an scFv-alkaline phosphatase fusion protein which was able to directly detect virally produced material without the need for additional reagents.
Original language | English (US) |
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Pages (from-to) | 60-69 |
Number of pages | 10 |
Journal | Journal of Immunological Methods |
Volume | 321 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 10 2007 |
Externally published | Yes |
Keywords
- Hantavirus
- Nucleocapsid protein
- Phage antibody
- Phage display
- Sin Nombre Virus
- scFv
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology