TY - JOUR
T1 - Scanning electronic microscopy images and energy-dispersive x-ray microanalysis of the stapes in otosclerosis and van der Hoeve syndrome
AU - Vallejo-Valdezate, Luis Angel
AU - Martín-Gil, Jesús
AU - José-Yacamán, Manuel
AU - Martín-Gil, Francisco Javier
AU - Gil-Carcedo, Luis Maria
PY - 2000
Y1 - 2000
N2 - Objective/Hypothesis: The objective of this study was to evaluate the morphological and microchemical changes that affect sclerotic stapes in otospongiosis and van der Hoeve syndrome. Methods: A scanning electron microscope equipped with an energy-dispersive x-ray analyzer was used in the experiments. Results: In otosclerosis, focal lesions are poorly mineralized, with low calcium salt and reduced calcium-to-phosphorus (Ca/P) ratio (1.9:1). This finding correlates with a spongiotic type of lesion and indicates unstable mineralization with possible change from hydroxyapatite to calcium triphosphate. In van der Hoeve syndrome the presence of magnesium in stapes suggests osteoclastic function stimulation. The osteoclasts secrete many protons, causing an acidified microenvironment. Brushite is formed, and Ca/P ratio decreases in comparison with that of control patients (2.0:1 vs. 2.6:1).
AB - Objective/Hypothesis: The objective of this study was to evaluate the morphological and microchemical changes that affect sclerotic stapes in otospongiosis and van der Hoeve syndrome. Methods: A scanning electron microscope equipped with an energy-dispersive x-ray analyzer was used in the experiments. Results: In otosclerosis, focal lesions are poorly mineralized, with low calcium salt and reduced calcium-to-phosphorus (Ca/P) ratio (1.9:1). This finding correlates with a spongiotic type of lesion and indicates unstable mineralization with possible change from hydroxyapatite to calcium triphosphate. In van der Hoeve syndrome the presence of magnesium in stapes suggests osteoclastic function stimulation. The osteoclasts secrete many protons, causing an acidified microenvironment. Brushite is formed, and Ca/P ratio decreases in comparison with that of control patients (2.0:1 vs. 2.6:1).
KW - Brushite
KW - Calcium-to-phosphorus ratio
KW - Hydroxyapatite
KW - Otosclerosis
KW - Scanning electron microscopy
KW - Stapes-otospongiosis
KW - Van der Hoeve syndrome
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U2 - 10.1097/00005537-200009000-00018
DO - 10.1097/00005537-200009000-00018
M3 - Article
C2 - 10983951
AN - SCOPUS:0033624382
SN - 0023-852X
VL - 110
SP - 1505
EP - 1510
JO - Laryngoscope
JF - Laryngoscope
IS - 9
ER -