Restriction fragment length polymorphism diversity in soybean

P. Keim, R. C. Shoemaker, R. G. Palmer

Research output: Contribution to journalArticlepeer-review

125 Scopus citations


Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers exhibited three alleles per locus, whereas all others had only two alleles. Thirty-five percent of the markers had rare alleles present in only 1 or 2 of the 58 accessions. Molecular diversity was least among cultivated soybeans and greatest between accessions of different soybean species such as Glycine max (L.) Merr. and G. soja Sieb. and Zucc. Principal component analysis was useful in reducing the multidimensional genotype data set and identifying genetic relationships.

Original languageEnglish (US)
Pages (from-to)786-792
Number of pages7
JournalTheoretical and Applied Genetics
Issue number6
StatePublished - Jun 1989


  • Glycine soja
  • Restriction fragment length polymorphism Glycine max

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics


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