Repressor for the sn-glycerol-3-phosphate regulon of Escherichia coli K-12: Cloning of the glpR gene and identification of its product

H. Schweizer, W. Boos, T. J. Larson

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The glpR gene encoding the repressor for the glp regulon of Escherichia coli was cloned from a library of HindIII DNA fragments established in bacteriophage lambda. Phages harboring glpR were isolated by selection for sn-glycerol-3-phosphate dehydrogenase function encoded by glpD, which is adjacent to glpR on the E. coli linkage map. Restriction endonuclease analysis and recloning of DNA fragments localized glpR to a 3-kilobase-pair EcoRI-SalI segment of DNA. Strains exhibiting constitutive expression of the glp operons were strongly repressed after introduction of multicopy plasmids containing the glpR gene. Analysis of proteins labeled in minicells harboring either glpR+ recombinant plasmids or a glpR::Tn5 derivative showed that the glpR gene product is a protein with an apparent molecular weight of 33,000.

Original languageEnglish (US)
Pages (from-to)563-566
Number of pages4
JournalJournal of Bacteriology
Volume161
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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