TY - JOUR
T1 - Rapid phytochemical analysis of birch (Betula) and poplar (Populus) foliage by near-infrared reflectance spectroscopy
AU - Rubert-Nason, Kennedy F.
AU - Holeski, Liza M.
AU - Couture, John J.
AU - Gusse, Adam
AU - Undersander, Daniel J.
AU - Lindroth, Richard L.
N1 - Funding Information:
Acknowledgments We thank Michael Hillstrom and Peter Ladwig for laboratory assistance, Kevin Silveira for technical assistance in the operation of the FOSS NIR spectrometer, Nicholas Keuler (UW-Madison CALS Statistical Consulting Services) for statistical consultation, and Timothy Meehan for the helpful discussion. Funding for this research was provided by the U.S. Department of Energy (Office of Science, BER) grant DE-FG02-06ER64232 and the National Science Foundation (grants DEB-0425908 and DEB-0841609).
PY - 2013/2
Y1 - 2013/2
N2 - Poplar (Populus) and birch (Betula) species are widely distributed throughout the northern hemisphere, where they are foundation species in forest ecosystems and serve as important sources of pulpwood. The ecology of these species is strongly linked to their foliar chemistry, creating demand for a rapid, inexpensive method to analyze phytochemistry. Our study demonstrates the feasibility of using near-infrared reflectance spectroscopy (NIRS) as an inexpensive, high-throughput tool for determining primary (e.g., nitrogen, sugars, starch) and secondary (e.g., tannins, phenolic glycosides) foliar chemistry of Populus and Betula species, and identifies conditions necessary for obtaining reliable quantitative data. We developed calibrations with high predictive power (residual predictive deviations ≤ 7.4) by relating phytochemical concentrations determined with classical analytical methods (e.g., spectrophotometric assays, liquid chromatography) to NIR spectra, using modified partial least squares regression. We determine that NIRS, although less sensitive and precise than classical methods for some compounds, provides useful predictions in a much faster, less expensive manner than do classical methods.
AB - Poplar (Populus) and birch (Betula) species are widely distributed throughout the northern hemisphere, where they are foundation species in forest ecosystems and serve as important sources of pulpwood. The ecology of these species is strongly linked to their foliar chemistry, creating demand for a rapid, inexpensive method to analyze phytochemistry. Our study demonstrates the feasibility of using near-infrared reflectance spectroscopy (NIRS) as an inexpensive, high-throughput tool for determining primary (e.g., nitrogen, sugars, starch) and secondary (e.g., tannins, phenolic glycosides) foliar chemistry of Populus and Betula species, and identifies conditions necessary for obtaining reliable quantitative data. We developed calibrations with high predictive power (residual predictive deviations ≤ 7.4) by relating phytochemical concentrations determined with classical analytical methods (e.g., spectrophotometric assays, liquid chromatography) to NIR spectra, using modified partial least squares regression. We determine that NIRS, although less sensitive and precise than classical methods for some compounds, provides useful predictions in a much faster, less expensive manner than do classical methods.
KW - Carbohydrate
KW - NIRS
KW - Nitrogen
KW - Phenolic glycoside
KW - Populus
KW - Tannin
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U2 - 10.1007/s00216-012-6513-6
DO - 10.1007/s00216-012-6513-6
M3 - Article
C2 - 23180073
AN - SCOPUS:84873749512
SN - 1618-2642
VL - 405
SP - 1333
EP - 1344
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 4
ER -