Raman spectra of porcine mitochondrial malate dehydrogenase

Raman spectra of porcine mitochondrial malate dehydrogenase have been collected and analysed. The existence of weak hydrogen bonds is indicated by the intensity ratio of the tyrosine doublet and a band is found which can be attributed to disulfide bonds. The observed frequencies in the amide I and III regions favor significant contributions from α‐helix and random conformations. An analysis of the intensities using standard methods predicts a very low β‐sheet content.