Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI

J. Paul Kirwan; Ty A. Gould; Herbert P. Schweizer; Scott W. Bearden; Robert C. Murphy; Mair E.A. Churchill

doi:10.1128/JB.188.2.784-788.2006

Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI

J. Paul Kirwan
, Ty A. Gould
, Herbert P. Schweizer
, Scott W. Bearden
, Robert C. Murphy
, Mair E.A. Churchill

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.

Original language	English (US)
Pages (from-to)	784-788
Number of pages	5
Journal	Journal of Bacteriology
Volume	188
Issue number	2
DOIs	https://doi.org/10.1128/JB.188.2.784-788.2006
State	Published - Jan 2006
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/JB.188.2.784-788.2006

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title = "Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI",

abstract = "The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.",

author = "Kirwan, \{J. Paul\} and Gould, \{Ty A.\} and Schweizer, \{Herbert P.\} and Bearden, \{Scott W.\} and Murphy, \{Robert C.\} and Churchill, \{Mair E.A.\}",

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AU - Kirwan, J. Paul

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AU - Schweizer, Herbert P.

AU - Bearden, Scott W.

AU - Murphy, Robert C.

AU - Churchill, Mair E.A.

PY - 2006/1

Y1 - 2006/1

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AB - The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.

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Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI

Abstract

ASJC Scopus subject areas

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