Quantitative multiplex detection of pathogen biomarkers on multichannel waveguides

Harshini Mukundan, Hongzhi Xie, Dominique Price, Jessica Z. Kubicek-Sutherland, W. Kevin Grace, Aaron S. Anderson, Jennifer S. Martinez, Nile Hartman, Basil I. Swanson

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


No single biomarker can accurately predict disease. An ideal biodetection technology should be capable of the quantitative, reproducible, and sensitive detection of a limited suite of such molecules. To this end, we have developed a multiplex biomarker assay for protective antigen and lethal factor of the Bacillus anthracis lethal toxin using semiconductor quantum dots as the fluorescence reporters on our waveguide-based biosensor platform. The platform is extendable to a wide array of biomarkers, facilitating rapid, quantitative, sensitive, and multiplex detection, better than achievable by conventional immunoassay. Our assay allows for the sensitive (limit of detection 1 pM each), specific (minimal nonspecific binding), and rapid (15 min) detection of these biomarkers in complex biological samples (e.g., serum). To address the issue of reproducibility in measurement and to increase our sample throughput, we have incorporated multichannel waveguides capable of simultaneous multiplex detection of biomarkers in three samples in quadruplicate. In this paper, we present the design, fabrication, and development of multichannel waveguides for the simultaneous detection of lethal factor and protective antigen in serum. Evaluation of the multichannel waveguide shows an excellent concordance with singlechannel data and effective, simultaneous, and reproducible measurement of lethal toxins in three samples.

Original languageEnglish (US)
Pages (from-to)136-144
Number of pages9
JournalAnalytical Chemistry
Issue number1
StatePublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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