TY - JOUR
T1 - Quantitative multiplex detection of pathogen biomarkers on multichannel waveguides
AU - Mukundan, Harshini
AU - Xie, Hongzhi
AU - Price, Dominique
AU - Kubicek-Sutherland, Jessica Z.
AU - Kevin Grace, W.
AU - Anderson, Aaron S.
AU - Martinez, Jennifer S.
AU - Hartman, Nile
AU - Swanson, Basil I.
PY - 2010
Y1 - 2010
N2 - No single biomarker can accurately predict disease. An ideal biodetection technology should be capable of the quantitative, reproducible, and sensitive detection of a limited suite of such molecules. To this end, we have developed a multiplex biomarker assay for protective antigen and lethal factor of the Bacillus anthracis lethal toxin using semiconductor quantum dots as the fluorescence reporters on our waveguide-based biosensor platform. The platform is extendable to a wide array of biomarkers, facilitating rapid, quantitative, sensitive, and multiplex detection, better than achievable by conventional immunoassay. Our assay allows for the sensitive (limit of detection 1 pM each), specific (minimal nonspecific binding), and rapid (15 min) detection of these biomarkers in complex biological samples (e.g., serum). To address the issue of reproducibility in measurement and to increase our sample throughput, we have incorporated multichannel waveguides capable of simultaneous multiplex detection of biomarkers in three samples in quadruplicate. In this paper, we present the design, fabrication, and development of multichannel waveguides for the simultaneous detection of lethal factor and protective antigen in serum. Evaluation of the multichannel waveguide shows an excellent concordance with singlechannel data and effective, simultaneous, and reproducible measurement of lethal toxins in three samples.
AB - No single biomarker can accurately predict disease. An ideal biodetection technology should be capable of the quantitative, reproducible, and sensitive detection of a limited suite of such molecules. To this end, we have developed a multiplex biomarker assay for protective antigen and lethal factor of the Bacillus anthracis lethal toxin using semiconductor quantum dots as the fluorescence reporters on our waveguide-based biosensor platform. The platform is extendable to a wide array of biomarkers, facilitating rapid, quantitative, sensitive, and multiplex detection, better than achievable by conventional immunoassay. Our assay allows for the sensitive (limit of detection 1 pM each), specific (minimal nonspecific binding), and rapid (15 min) detection of these biomarkers in complex biological samples (e.g., serum). To address the issue of reproducibility in measurement and to increase our sample throughput, we have incorporated multichannel waveguides capable of simultaneous multiplex detection of biomarkers in three samples in quadruplicate. In this paper, we present the design, fabrication, and development of multichannel waveguides for the simultaneous detection of lethal factor and protective antigen in serum. Evaluation of the multichannel waveguide shows an excellent concordance with singlechannel data and effective, simultaneous, and reproducible measurement of lethal toxins in three samples.
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U2 - 10.1021/ac901497g
DO - 10.1021/ac901497g
M3 - Article
C2 - 20000585
AN - SCOPUS:75749093442
SN - 0003-2700
VL - 82
SP - 136
EP - 144
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 1
ER -