Purification and characterization of rhodobactin: A mixed ligand siderophore from Rhodococcus rhodochrous strain OFS

Suraj Dhungana, Ryszard Michalczyk, Hakim Boukhalfa, Joseph G. Lack, Andrew T. Koppisch, Jason M. Fairlee, Mitchell T. Johnson, Christy E. Ruggiero, Seth G. John, Matthew M. Cox, Cindy C. Browder, Jennifer H. Forsythe, Laura A. Vanderberg, Mary P. Neu, Larry E. Hersman

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D 1H, 13C and 15N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 μM to 2.0 μM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation.

Original languageEnglish (US)
Pages (from-to)853-867
Number of pages15
Issue number6
StatePublished - Dec 2007


  • Catecholate
  • Hydroxamate
  • Rhodobactin
  • Rhodococcus
  • Siderophore

ASJC Scopus subject areas

  • Biomaterials
  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • Metals and Alloys


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