PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Dongru Qiu; F. Heath Damron; Takehiko Mima; Herbert P. Schweizer; Hongwei D. Yu

doi:10.1128/AEM.01369-08

P_BAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Dongru Qiu
, F. Heath Damron
, Takehiko Mima
, Herbert P. Schweizer
, Hongwei D. Yu

Research output: Contribution to journal › Article › peer-review

213 Scopus citations

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P_BAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Original language	English (US)
Pages (from-to)	7422-7426
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	23
DOIs	https://doi.org/10.1128/AEM.01369-08
State	Published - Dec 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria",

abstract = "We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.",

author = "Dongru Qiu and Damron, \{F. Heath\} and Takehiko Mima and Schweizer, \{Herbert P.\} and Yu, \{Hongwei D.\}",

year = "2008",

month = dec,

doi = "10.1128/AEM.01369-08",

language = "English (US)",

volume = "74",

pages = "7422--7426",

journal = "Applied and Environmental Microbiology",

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publisher = "American Society for Microbiology",

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T1 - PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

AU - Qiu, Dongru

AU - Damron, F. Heath

AU - Mima, Takehiko

AU - Schweizer, Herbert P.

AU - Yu, Hongwei D.

PY - 2008/12

Y1 - 2008/12

N2 - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

AB - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

UR - https://www.scopus.com/pages/publications/57149099185

UR - https://www.scopus.com/pages/publications/57149099185#tab=citedBy

U2 - 10.1128/AEM.01369-08

DO - 10.1128/AEM.01369-08

M3 - Article

C2 - 18849445

AN - SCOPUS:57149099185

SN - 0099-2240

VL - 74

SP - 7422

EP - 7426

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 23

ER -

P_BAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Abstract

ASJC Scopus subject areas

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