PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Dongru Qiu; F. Heath Damron; Takehiko Mima; Herbert P. Schweizer; Hongwei D. Yu

doi:10.1128/AEM.01369-08

P_BAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Dongru Qiu, F. Heath Damron, Takehiko Mima, Herbert P. Schweizer, Hongwei D. Yu

Research output: Contribution to journal › Article › peer-review

196 Scopus citations

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P_BAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Original language	English (US)
Pages (from-to)	7422-7426
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	23
DOIs	https://doi.org/10.1128/AEM.01369-08
State	Published - Dec 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria",

abstract = "We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.",

author = "Dongru Qiu and Damron, {F. Heath} and Takehiko Mima and Schweizer, {Herbert P.} and Yu, {Hongwei D.}",

year = "2008",

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AU - Qiu, Dongru

AU - Damron, F. Heath

AU - Mima, Takehiko

AU - Schweizer, Herbert P.

AU - Yu, Hongwei D.

PY - 2008/12

Y1 - 2008/12

N2 - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

AB - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

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SN - 0099-2240

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ER -

P_BAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Abstract

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