Pseudomonas aeruginosa mexT is an indicator of PAO1 strain integrity

Eric D. LoVullo, Herbert P. Schweizer

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Laboratory research with Pseudomonas aeruginosa commonly involves the prototype strain PAO1. There is continued concern that PAO1 sublines maintained and propagated in the same laboratory or different laboratories exhibit genetic and phenotypic variability that may affect the reproducibility and validity of research. Whole-genome sequencing and other research identified the mexT locus as a mutational hotspot, but the explication of the diverse mutations present in the various sublines and consequences remained rather cursory. Here we present evidence that MexT sequence diversity is a predictor of PAO1 lineage integrity and define the protein’s prototype sequence.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalJournal of Medical Microbiology
Volume69
Issue number1
DOIs
StatePublished - 2020
Externally publishedYes

Keywords

  • Lineages
  • MexT diversity
  • Pseudomonas aeruginosa
  • Strain PAO1

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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