Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius

F. G. Monroy; C. J.R. Cayzer; J. H. Adams; C. Dobson

doi:10.1016/0020-7519(89)90031-3

Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius

F. G. Monroy, C. J.R. Cayzer, J. H. Adams, C. Dobson

Biological Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius. International Journal for Parasitology 19: 129-131. Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.

Original language	English (US)
Pages (from-to)	129-131
Number of pages	3
Journal	International Journal for Parasitology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/0020-7519(89)90031-3
State	Published - Feb 1989

Keywords

Nematoda
Nematospiroides dubius
enzymes
excretory-secretory products
inhibitors

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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10.1016/0020-7519(89)90031-3

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title = "Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius",

abstract = "Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius. International Journal for Parasitology 19: 129-131. Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.",

keywords = "Nematoda, Nematospiroides dubius, enzymes, excretory-secretory products, inhibitors",

author = "Monroy, {F. G.} and Cayzer, {C. J.R.} and Adams, {J. H.} and C. Dobson",

note = "Funding Information: Acknowfedgemenrs-This work was supportedb y a grant from the AustralianR esearchG rantS chemeW. e thankD r I. J. East for helpfuld iscussion.",

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AU - Monroy, F. G.

AU - Cayzer, C. J.R.

AU - Adams, J. H.

AU - Dobson, C.

N1 - Funding Information: Acknowfedgemenrs-This work was supportedb y a grant from the AustralianR esearchG rantS chemeW. e thankD r I. J. East for helpfuld iscussion.

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N2 - Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius. International Journal for Parasitology 19: 129-131. Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.

AB - Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius. International Journal for Parasitology 19: 129-131. Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.

KW - Nematoda

KW - Nematospiroides dubius

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KW - excretory-secretory products

KW - inhibitors

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Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius

Abstract

Keywords

ASJC Scopus subject areas

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