Proper Care and Feeding of Coccidioides: A Laboratorian's Guide to Cultivating the Dimorphic Stages of C. immitis and C. posadasii

Heather L. Mead, Marley C.Caballero Van Dyke, Bridget M. Barker

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Coccidioidomycosis (“Valley fever”) is caused by Coccidioides immitis and C. posadasii. These fungi are thermally dimorphic, cycling between mycelia and arthroconidia in the environment and converting into spherules and endospores within a host. Coccidioides can cause a broad spectrum of disease that can be difficult to treat. There has been a steady increase in disease, with an estimated 350,000 new infections per year in the United States. With the increase in disease and difficulty in treatment, there is an unmet need to increase research in basic biology and identify new treatments, diagnostics, and vaccine candidates. Here, we describe protocols required in any Coccidioides laboratory, such as growing, harvesting, and storing the different stages of this dimorphic fungal pathogen.

Original languageEnglish (US)
Article numbere113
JournalCurrent Protocols in Microbiology
Volume58
Issue number1
DOIs
StatePublished - Sep 1 2020

Keywords

  • arthroconidia
  • BSL-3
  • Coccidioides
  • dimorphic fungi
  • endospores
  • mycelia
  • spherules

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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