Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

Jan Sunner; Recep Avci; Lee Richards; Gary Groenewold; Jani Ingram; Megan Arthun

doi:10.1016/S0167-7012(03)00041-1

Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

Jan Sunner, Recep Avci, Lee Richards, Gary Groenewold, Jani Ingram, Megan Arthun

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

Original language	English (US)
Pages (from-to)	285-287
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	54
Issue number	2
DOIs	https://doi.org/10.1016/S0167-7012(03)00041-1
State	Published - Aug 1 2003

Keywords

Dehydration
Electron microscopy
Fixation
IR
Imaging
Infrared laser
Morphology
SEM
Yeast cells

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/S0167-7012(03)00041-1

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title = "Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation",

abstract = "As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.",

keywords = "Dehydration, Electron microscopy, Fixation, IR, Imaging, Infrared laser, Morphology, SEM, Yeast cells",

author = "Jan Sunner and Recep Avci and Lee Richards and Gary Groenewold and Jani Ingram and Megan Arthun",

note = "Funding Information: We wish to thank Jon Eckberg for help in setting up the laser system. Cell cultures were supplied by Nurdan Yurt and Mark Pasmore. The help of ICAL personnel with SEM operations are greatly appreciated. This work was funded by DOE-INRA Contract no. MSU001.",

year = "2003",

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doi = "10.1016/S0167-7012(03)00041-1",

language = "English (US)",

volume = "54",

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journal = "Journal of Microbiological Methods",

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TY - JOUR

T1 - Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

AU - Sunner, Jan

AU - Avci, Recep

AU - Richards, Lee

AU - Groenewold, Gary

AU - Ingram, Jani

AU - Arthun, Megan

N1 - Funding Information: We wish to thank Jon Eckberg for help in setting up the laser system. Cell cultures were supplied by Nurdan Yurt and Mark Pasmore. The help of ICAL personnel with SEM operations are greatly appreciated. This work was funded by DOE-INRA Contract no. MSU001.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

AB - As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

KW - Dehydration

KW - Electron microscopy

KW - Fixation

KW - IR

KW - Imaging

KW - Infrared laser

KW - Morphology

KW - SEM

KW - Yeast cells

UR - https://www.scopus.com/pages/publications/0038730521

UR - https://www.scopus.com/inward/citedby.url?scp=0038730521&partnerID=8YFLogxK

U2 - 10.1016/S0167-7012(03)00041-1

DO - 10.1016/S0167-7012(03)00041-1

M3 - Article

C2 - 12782385

AN - SCOPUS:0038730521

SN - 0167-7012

VL - 54

SP - 285

EP - 287

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

Abstract

Keywords

ASJC Scopus subject areas

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