Abstract
As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.
Original language | English (US) |
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Pages (from-to) | 285-287 |
Number of pages | 3 |
Journal | Journal of Microbiological Methods |
Volume | 54 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1 2003 |
Keywords
- Dehydration
- Electron microscopy
- Fixation
- IR
- Imaging
- Infrared laser
- Morphology
- SEM
- Yeast cells
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)