TY - JOUR
T1 - Presence and Diversity of Streptomyces in Dendroctonus and Sympatric Bark Beetle Galleries Across North America
AU - Hulcr, Jiri
AU - Adams, Aaron S.
AU - Raffa, Kenneth
AU - Hofstetter, Richard W.
AU - Klepzig, Kier D.
AU - Currie, Cameron R.
N1 - Funding Information:
This project would not be possible without the assistance of many colleagues and field assistants. We thank Brian Strom, Paul Merten, Jason Moan, Jesse Pfammatter, Anthony Cognato, and Andrea Lucky for their assistance with collecting. We thank Rob Dugenske, Joe Moeller, Laura Schwab, and many other members of the Currie lab for their help with laboratory work. The project was funded by the USDA Forest Service Southern Research Station, the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE-FC02-07ER64494), and NSF-Microbial Observatories (MCB-0702025).
PY - 2011/5
Y1 - 2011/5
N2 - Recent studies have revealed several examples of intimate associations between insects and Actinobacteria, including the Southern Pine Beetle Dendroctonus frontalis and the Spruce Beetle Dendroctonus rufipennis. Here, we surveyed Streptomyces Actinobacteria co-occurring with 10 species of Dendroctonus bark beetles across the United States, using both phylogenetic and community ecology approaches. From these 10 species, and 19 other scolytine beetles that occur in the same trees, we obtained 154 Streptomyces-like isolates and generated 16S sequences from 134 of those. Confirmed 16S sequences of Streptomyces were binned into 36 distinct strains using a threshold of 0.2% sequence divergence. The 16S rDNA phylogeny of all isolates does not correlate with the distribution of strains among beetle species, localities, or parts of the beetles or their galleries. However, we identified three Streptomyces strains occurring repeatedly on Dendroctonus beetles and in their galleries. Identity of these isolates was corroborated using a house-keeping gene sequence (efTu). These strains are not confined to a certain species of beetle, locality, or part of the beetle or their galleries. However, their role as residents in the woodboring insect niche is supported by the repeated association of their 16S and efTu from across the continent, and also having been reported in studies of other subcortical insects.
AB - Recent studies have revealed several examples of intimate associations between insects and Actinobacteria, including the Southern Pine Beetle Dendroctonus frontalis and the Spruce Beetle Dendroctonus rufipennis. Here, we surveyed Streptomyces Actinobacteria co-occurring with 10 species of Dendroctonus bark beetles across the United States, using both phylogenetic and community ecology approaches. From these 10 species, and 19 other scolytine beetles that occur in the same trees, we obtained 154 Streptomyces-like isolates and generated 16S sequences from 134 of those. Confirmed 16S sequences of Streptomyces were binned into 36 distinct strains using a threshold of 0.2% sequence divergence. The 16S rDNA phylogeny of all isolates does not correlate with the distribution of strains among beetle species, localities, or parts of the beetles or their galleries. However, we identified three Streptomyces strains occurring repeatedly on Dendroctonus beetles and in their galleries. Identity of these isolates was corroborated using a house-keeping gene sequence (efTu). These strains are not confined to a certain species of beetle, locality, or part of the beetle or their galleries. However, their role as residents in the woodboring insect niche is supported by the repeated association of their 16S and efTu from across the continent, and also having been reported in studies of other subcortical insects.
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U2 - 10.1007/s00248-010-9797-0
DO - 10.1007/s00248-010-9797-0
M3 - Article
C2 - 21249352
AN - SCOPUS:79957515308
SN - 0095-3628
VL - 61
SP - 759
EP - 768
JO - Microbial Ecology
JF - Microbial Ecology
IS - 4
ER -