Physical and genetic structure of the glpD-malT interval of the Escherichia coli K-12 chromosome - Identification of two new structural genes of the glp-regulon

Herbert Schweizer, Gaye Sweet, Timothy J. Larson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A transducing λ phage carrying glpD″lacZ, glpR, and malT was isolated from a strain harboring a glpD″lacZ fusion. Comparison of restriction endonuclease cleavage patterns of DNA isolated from this phage with that of the previously cloned malT region (Raibaud and Schwartz 1980) facilitated the construction of recombinant plasmids carrying different portions of the glpD-malT region. Results of minicell analysis and complementation studies showed that this region of the chromosome encodes at least five polypeptides. These included the previously identified glpD, glpR, and malT gene products. In addition, two new structural genes of the glp regulon (glpE and glpG) located between the glpD and glpR genes were identified. Hybrid plasmids carrying glpD″lacZ and glpR″lacZ fusions were constructed. Restriction endonuclease cleavage analysis of these two plasmids demonstrated that glpD and glpR are divergently transcribed

Original languageEnglish (US)
Pages (from-to)488-492
Number of pages5
JournalMGG Molecular & General Genetics
Volume202
Issue number3
DOIs
StatePublished - Mar 1986
Externally publishedYes

Keywords

  • E. coli K12
  • glpD-malT interval
  • New glp genes
  • Physical mapping

ASJC Scopus subject areas

  • Genetics

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