Photothermal nanoblade for large cargo delivery into mammalian cells

T. H. Wu; T. Teslaa; S. Kalim; C. T. French; S. Moghadam; R. Wall; J. F. Miller; O. N. Witte; M. A. Teitell; P. Y. Chiou

doi:10.1109/TRANSDUCERS.2011.5969731

Photothermal nanoblade for large cargo delivery into mammalian cells

T. H. Wu
, T. Teslaa
, S. Kalim
, C. T. French
, S. Moghadam
, R. Wall
, J. F. Miller
, O. N. Witte
, M. A. Teitell
, P. Y. Chiou

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest laser pulse energy and convert it into a localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. Integrating the metallic nanostructure with a micropipette, the nanoblade generates a micron-sized membrane access port for delivering concentrated cargo (5×10⁸ bacteria/ml) with high efficiency (46%) and cell viability (>90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, 200 nm polystyrene beads to 2 μm bacteria have been delivered into multiple cell types.

Original language	English (US)
Title of host publication	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages	1813-1816
Number of pages	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969731
State	Published - 2011
Externally published	Yes
Event	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China Duration: Jun 5 2011 → Jun 9 2011

Publication series

Name	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/Territory	China
City	Beijing
Period	6/5/11 → 6/9/11

Keywords

Photothermal
cell surgery
pulsed laser
transfection

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2011.5969731

Cite this

Wu, T. H., Teslaa, T., Kalim, S., French, C. T., Moghadam, S., Wall, R., Miller, J. F., Witte, O. N., Teitell, M. A., & Chiou, P. Y. (2011). Photothermal nanoblade for large cargo delivery into mammalian cells. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 1813-1816). Article 5969731 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969731

Photothermal nanoblade for large cargo delivery into mammalian cells. / Wu, T. H.; Teslaa, T.; Kalim, S. et al.
2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 1813-1816 5969731 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Wu, TH, Teslaa, T, Kalim, S, French, CT, Moghadam, S, Wall, R, Miller, JF, Witte, ON, Teitell, MA & Chiou, PY 2011, Photothermal nanoblade for large cargo delivery into mammalian cells. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969731, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp. 1813-1816, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 6/5/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969731

Wu TH, Teslaa T, Kalim S, French CT, Moghadam S, Wall R et al. Photothermal nanoblade for large cargo delivery into mammalian cells. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 1813-1816. 5969731. (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). doi: 10.1109/TRANSDUCERS.2011.5969731

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title = "Photothermal nanoblade for large cargo delivery into mammalian cells",

abstract = "We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest laser pulse energy and convert it into a localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. Integrating the metallic nanostructure with a micropipette, the nanoblade generates a micron-sized membrane access port for delivering concentrated cargo (5×108 bacteria/ml) with high efficiency (46\%) and cell viability (>90\%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, 200 nm polystyrene beads to 2 μm bacteria have been delivered into multiple cell types.",

keywords = "Photothermal, cell surgery, pulsed laser, transfection",

author = "Wu, \{T. H.\} and T. Teslaa and S. Kalim and French, \{C. T.\} and S. Moghadam and R. Wall and Miller, \{J. F.\} and Witte, \{O. N.\} and Teitell, \{M. A.\} and Chiou, \{P. Y.\}",

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AU - Teslaa, T.

AU - Kalim, S.

AU - French, C. T.

AU - Moghadam, S.

AU - Wall, R.

AU - Miller, J. F.

AU - Witte, O. N.

AU - Teitell, M. A.

AU - Chiou, P. Y.

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AB - We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest laser pulse energy and convert it into a localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. Integrating the metallic nanostructure with a micropipette, the nanoblade generates a micron-sized membrane access port for delivering concentrated cargo (5×108 bacteria/ml) with high efficiency (46%) and cell viability (>90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, 200 nm polystyrene beads to 2 μm bacteria have been delivered into multiple cell types.

KW - Photothermal

KW - cell surgery

KW - pulsed laser

KW - transfection

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Y2 - 5 June 2011 through 9 June 2011

ER -

Photothermal nanoblade for large cargo delivery into mammalian cells

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this