TY - JOUR
T1 - Photothermal nanoblade for large cargo delivery into mammalian cells
AU - Wu, Ting Hsiang
AU - Teslaa, Tara
AU - Kalim, Sheraz
AU - French, Christopher T.
AU - Moghadam, Shahriar
AU - Wall, Randolph
AU - Miller, Jeffery F.
AU - Witte, Owen N.
AU - Teitell, Michael A.
AU - Chiou, Pei Yu
PY - 2011/2/15
Y1 - 2011/2/15
N2 - It is difficult to achieve controlled cutting of elastic, mechanically fragile, and rapidly resealing mammalian cell membranes. Here, we report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized explosive vapor bubble, which rapidly punctures a lightly contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The cavitation bubble pattern is controlled by the metallic structure configuration and laser pulse duration and energy. Integration of the metallic nanostructure with a micropipet, the nanoblade generates a micrometer-sized membrane access port for delivering highly concentrated cargo (5 × 108 live bacteria/mL) with high efficiency (46%) and cell viability (>90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, RNA, 200 nm polystyrene beads, to 2 μ bacteria have also been delivered into multiple mammalian cell types. Overall, the photothermal nanoblade is a new approach for delivering difficult cargo into mammalian cells.
AB - It is difficult to achieve controlled cutting of elastic, mechanically fragile, and rapidly resealing mammalian cell membranes. Here, we report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized explosive vapor bubble, which rapidly punctures a lightly contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The cavitation bubble pattern is controlled by the metallic structure configuration and laser pulse duration and energy. Integration of the metallic nanostructure with a micropipet, the nanoblade generates a micrometer-sized membrane access port for delivering highly concentrated cargo (5 × 108 live bacteria/mL) with high efficiency (46%) and cell viability (>90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, RNA, 200 nm polystyrene beads, to 2 μ bacteria have also been delivered into multiple mammalian cell types. Overall, the photothermal nanoblade is a new approach for delivering difficult cargo into mammalian cells.
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U2 - 10.1021/ac102532w
DO - 10.1021/ac102532w
M3 - Article
C2 - 21247066
AN - SCOPUS:79951658371
SN - 0003-2700
VL - 83
SP - 1321
EP - 1327
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 4
ER -