@inproceedings{e5cde9a232ee41d99901a05fbe593cb1,
title = "Photothermal nanoblade for large cargo delivery into mammalian cells",
abstract = "We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest laser pulse energy and convert it into a localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. Integrating the metallic nanostructure with a micropipette, the nanoblade generates a micron-sized membrane access port for delivering concentrated cargo (5×108 bacteria/ml) with high efficiency (46%) and cell viability (>90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, 200 nm polystyrene beads to 2 μm bacteria have been delivered into multiple cell types.",
keywords = "Photothermal, cell surgery, pulsed laser, transfection",
author = "Wu, {T. H.} and T. Teslaa and S. Kalim and French, {C. T.} and S. Moghadam and R. Wall and Miller, {J. F.} and Witte, {O. N.} and Teitell, {M. A.} and Chiou, {P. Y.}",
year = "2011",
doi = "10.1109/TRANSDUCERS.2011.5969731",
language = "English (US)",
isbn = "9781457701573",
series = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11",
pages = "1813--1816",
booktitle = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11",
note = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 ; Conference date: 05-06-2011 Through 09-06-2011",
}