Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

Andrew T. Koppisch; Cindy C. Browder; Amanda L. Moe; Jacob T. Shelley; Blaine A. Kinkel; Larry E. Hersman; Srinivas Iyer; Christy E. Ruggiero

doi:10.1007/s10534-005-1782-6

Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

Andrew T. Koppisch, Cindy C. Browder, Amanda L. Moe, Jacob T. Shelley, Blaine A. Kinkel, Larry E. Hersman, Srinivas Iyer, Christy E. Ruggiero

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of ¹H and ¹³C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

Original language	English (US)
Pages (from-to)	577-585
Number of pages	9
Journal	BioMetals
Volume	18
Issue number	6
DOIs	https://doi.org/10.1007/s10534-005-1782-6
State	Published - Dec 2005

Keywords

Bacillibactin
Bacillus anthracis
Iron
Petrobactin
Siderophores

ASJC Scopus subject areas

Biomaterials
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

Access to Document

10.1007/s10534-005-1782-6

Cite this

@article{537a8537336a4f2b91f4008d8919df17,

title = "Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation",

abstract = "The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.",

keywords = "Bacillibactin, Bacillus anthracis, Iron, Petrobactin, Siderophores",

author = "Koppisch, {Andrew T.} and Browder, {Cindy C.} and Moe, {Amanda L.} and Shelley, {Jacob T.} and Kinkel, {Blaine A.} and Hersman, {Larry E.} and Srinivas Iyer and Ruggiero, {Christy E.}",

note = "Funding Information: A.T.K. is supported by a LANL LDRD-ER Director{\textquoteright}s Fellowship. A.L.M. is supported by the NSF-REU Bioscience and Biotechnology PUSH program for northern New Mexico. We thank Drs. Suraj Dhungana, Jen Martinez, and Hakim Boukhalfa for helpful discussions. This work was supported by a Laboratory Directed Research and Development ER grant. Los Almos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36. Boukhalfa for helpful discussions. This work was supported by a Laboratory Directed Research and Development ER grant. Los Alamos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36.",

year = "2005",

month = dec,

doi = "10.1007/s10534-005-1782-6",

language = "English (US)",

volume = "18",

pages = "577--585",

journal = "BioMetals",

issn = "0966-0844",

publisher = "Springer Netherlands",

number = "6",

}

TY - JOUR

T1 - Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

AU - Koppisch, Andrew T.

AU - Browder, Cindy C.

AU - Moe, Amanda L.

AU - Shelley, Jacob T.

AU - Kinkel, Blaine A.

AU - Hersman, Larry E.

AU - Iyer, Srinivas

AU - Ruggiero, Christy E.

N1 - Funding Information: A.T.K. is supported by a LANL LDRD-ER Director’s Fellowship. A.L.M. is supported by the NSF-REU Bioscience and Biotechnology PUSH program for northern New Mexico. We thank Drs. Suraj Dhungana, Jen Martinez, and Hakim Boukhalfa for helpful discussions. This work was supported by a Laboratory Directed Research and Development ER grant. Los Almos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36. Boukhalfa for helpful discussions. This work was supported by a Laboratory Directed Research and Development ER grant. Los Alamos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36.

PY - 2005/12

Y1 - 2005/12

N2 - The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

AB - The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

KW - Bacillibactin

KW - Bacillus anthracis

KW - Iron

KW - Petrobactin

KW - Siderophores

UR - http://www.scopus.com/inward/record.url?scp=29744459466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29744459466&partnerID=8YFLogxK

U2 - 10.1007/s10534-005-1782-6

DO - 10.1007/s10534-005-1782-6

M3 - Article

C2 - 16388397

AN - SCOPUS:29744459466

SN - 0966-0844

VL - 18

SP - 577

EP - 585

JO - BioMetals

JF - BioMetals

IS - 6

ER -

Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this