Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria

Andrew T. Koppisch; Suraj Dhungana; Karen K. Hill; Hakim Boukhalfa; Henry S. Heine; Leslie A. Colip; Raymond B. Romero; Yulin Shou; Lawrence O. Ticknor; Babetta L. Marrone; Larry E. Hersman; Srinivas Iyer; Christy E. Ruggiero

doi:10.1007/s10534-008-9144-9

Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria

Andrew T. Koppisch, Suraj Dhungana, Karen K. Hill, Hakim Boukhalfa, Henry S. Heine, Leslie A. Colip, Raymond B. Romero, Yulin Shou, Lawrence O. Ticknor, Babetta L. Marrone, Larry E. Hersman, Srinivas Iyer, Christy E. Ruggiero

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore's biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis. Thus, a common hypothesis regarding siderophore production in Bacillus species is that petrobactin production is exclusive to pathogenic isolates. In order to test this hypothesis, siderophores produced by 106 strains of an in-house library of the Bacillus cereus sensu lato group were isolated and identified using a MALDI-TOF-MS assay. Strains were selected from a previously defined phylogenetic tree of this group in order to include both known pathogens and innocuous strains. Petrobactin is produced by pathogenic strains and innocuous isolates alike, and thus is not itself indicative of virulence.

Original language	English (US)
Pages (from-to)	581-589
Number of pages	9
Journal	BioMetals
Volume	21
Issue number	5
DOIs	https://doi.org/10.1007/s10534-008-9144-9
State	Published - Oct 2008

Keywords

Bacillus anthracis
Bacillus cereus
Bacillus thuringiensis
Iron
Siderophores

ASJC Scopus subject areas

Biomaterials
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

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10.1007/s10534-008-9144-9

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Koppisch, A. T., Dhungana, S., Hill, K. K., Boukhalfa, H., Heine, H. S., Colip, L. A., Romero, R. B., Shou, Y., Ticknor, L. O., Marrone, B. L., Hersman, L. E., Iyer, S., & Ruggiero, C. E. (2008). Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria. BioMetals, 21(5), 581-589. https://doi.org/10.1007/s10534-008-9144-9

@article{93b4fa5357c04ffbb7856abd8c50bf48,

title = "Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria",

abstract = "Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore's biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis. Thus, a common hypothesis regarding siderophore production in Bacillus species is that petrobactin production is exclusive to pathogenic isolates. In order to test this hypothesis, siderophores produced by 106 strains of an in-house library of the Bacillus cereus sensu lato group were isolated and identified using a MALDI-TOF-MS assay. Strains were selected from a previously defined phylogenetic tree of this group in order to include both known pathogens and innocuous strains. Petrobactin is produced by pathogenic strains and innocuous isolates alike, and thus is not itself indicative of virulence.",

keywords = "Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Iron, Siderophores",

author = "Koppisch, {Andrew T.} and Suraj Dhungana and Hill, {Karen K.} and Hakim Boukhalfa and Heine, {Henry S.} and Colip, {Leslie A.} and Romero, {Raymond B.} and Yulin Shou and Ticknor, {Lawrence O.} and Marrone, {Babetta L.} and Hersman, {Larry E.} and Srinivas Iyer and Ruggiero, {Christy E.}",

note = "Funding Information: Acknowledgements R.B.R. is supported by the NSF-REU Bioscience and Biotechnology PUSH program for northern New Mexico. We thank Drs. David Fox and Cindy Browder for helpful discussions. This work was supported by a Laboratory Directed Research and Development exploratory research grant. Los Alamos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36.",

year = "2008",

month = oct,

doi = "10.1007/s10534-008-9144-9",

language = "English (US)",

volume = "21",

pages = "581--589",

journal = "BioMetals",

issn = "0966-0844",

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number = "5",

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T1 - Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria

AU - Koppisch, Andrew T.

AU - Dhungana, Suraj

AU - Hill, Karen K.

AU - Boukhalfa, Hakim

AU - Heine, Henry S.

AU - Colip, Leslie A.

AU - Romero, Raymond B.

AU - Shou, Yulin

AU - Ticknor, Lawrence O.

AU - Marrone, Babetta L.

AU - Hersman, Larry E.

AU - Iyer, Srinivas

AU - Ruggiero, Christy E.

N1 - Funding Information: Acknowledgements R.B.R. is supported by the NSF-REU Bioscience and Biotechnology PUSH program for northern New Mexico. We thank Drs. David Fox and Cindy Browder for helpful discussions. This work was supported by a Laboratory Directed Research and Development exploratory research grant. Los Alamos National Laboratory is operated by the University of California for the U.S. Department of Energy under contract W-7405-ENG-36.

PY - 2008/10

Y1 - 2008/10

N2 - Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore's biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis. Thus, a common hypothesis regarding siderophore production in Bacillus species is that petrobactin production is exclusive to pathogenic isolates. In order to test this hypothesis, siderophores produced by 106 strains of an in-house library of the Bacillus cereus sensu lato group were isolated and identified using a MALDI-TOF-MS assay. Strains were selected from a previously defined phylogenetic tree of this group in order to include both known pathogens and innocuous strains. Petrobactin is produced by pathogenic strains and innocuous isolates alike, and thus is not itself indicative of virulence.

AB - Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore's biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis. Thus, a common hypothesis regarding siderophore production in Bacillus species is that petrobactin production is exclusive to pathogenic isolates. In order to test this hypothesis, siderophores produced by 106 strains of an in-house library of the Bacillus cereus sensu lato group were isolated and identified using a MALDI-TOF-MS assay. Strains were selected from a previously defined phylogenetic tree of this group in order to include both known pathogens and innocuous strains. Petrobactin is produced by pathogenic strains and innocuous isolates alike, and thus is not itself indicative of virulence.

KW - Bacillus anthracis

KW - Bacillus cereus

KW - Bacillus thuringiensis

KW - Iron

KW - Siderophores

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JO - BioMetals

JF - BioMetals

IS - 5

ER -

Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria

Abstract

Keywords

ASJC Scopus subject areas

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