Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

Cliff S. Han; Gary Xie; Jean F. Challacombe; Michael R. Altherr; Smriti S. Bhotika; David Bruce; Connie S. Campbell; Mary L. Campbell; Jin Chen; Olga Chertkov; Cathy Cleland; Mira Dimitrijevic; Norman A. Doggett; John J. Fawcett; Tijana Glavina; Lynne A. Goodwin; Karen K. Hill; Penny Hitchcock; Paul J. Jackson; Paul Keim; Avinash Ramesh Kewalramani; Jon Longmire; Susan Lucas; Stephanie Malfatti; Kim McMurry; Linda J. Meincke; Monica Misra; Bernice L. Moseman; Mark Mundt; A. Christine Munk; Richard T. Okinaka; B. Parson-Quintana; Lee Philip Reilly; Paul Richardson; Donna L. Robinson; Eddy Rubin; Elizabeth Saunders; Roxanne Tapia; Judith G. Tesmer; Nina Thayer; Linda S. Thompson; Hope Tice; Lawrence O. Ticknor; Patti L. Wills; Thomas S. Brettin; Paul Gilna

doi:10.1128/JB.188.9.3382-3390.2006

Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

Cliff S. Han, Gary Xie, Jean F. Challacombe, Michael R. Altherr, Smriti S. Bhotika, David Bruce, Connie S. Campbell, Mary L. Campbell, Jin Chen, Olga Chertkov, Cathy Cleland, Mira Dimitrijevic, Norman A. Doggett, John J. Fawcett, Tijana Glavina, Lynne A. Goodwin, Karen K. Hill, Penny Hitchcock, Paul J. Jackson, Paul KeimAvinash Ramesh Kewalramani, Jon Longmire, Susan Lucas, Stephanie Malfatti, Kim McMurry, Linda J. Meincke, Monica Misra, Bernice L. Moseman, Mark Mundt, A. Christine Munk, Richard T. Okinaka, B. Parson-Quintana, Lee Philip Reilly, Paul Richardson, Donna L. Robinson, Eddy Rubin, Elizabeth Saunders, Roxanne Tapia, Judith G. Tesmer, Nina Thayer, Linda S. Thompson, Hope Tice, Lawrence O. Ticknor, Patti L. Wills, Thomas S. Brettin, Paul Gilna

Biological Sciences

Research output: Contribution to journal › Article › peer-review

181 Scopus citations

Abstract

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

Original language	English (US)
Pages (from-to)	3382-3390
Number of pages	9
Journal	Journal of Bacteriology
Volume	188
Issue number	9
DOIs	https://doi.org/10.1128/JB.188.9.3382-3390.2006
State	Published - May 2006

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/JB.188.9.3382-3390.2006

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Han, C. S., Xie, G., Challacombe, J. F., Altherr, M. R., Bhotika, S. S., Bruce, D., Campbell, C. S., Campbell, M. L., Chen, J., Chertkov, O., Cleland, C., Dimitrijevic, M., Doggett, N. A., Fawcett, J. J., Glavina, T., Goodwin, L. A., Hill, K. K., Hitchcock, P., Jackson, P. J., ... Gilna, P. (2006). Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. Journal of Bacteriology, 188(9), 3382-3390. https://doi.org/10.1128/JB.188.9.3382-3390.2006

Han, CS, Xie, G, Challacombe, JF, Altherr, MR, Bhotika, SS, Bruce, D, Campbell, CS, Campbell, ML, Chen, J, Chertkov, O, Cleland, C, Dimitrijevic, M, Doggett, NA, Fawcett, JJ, Glavina, T, Goodwin, LA, Hill, KK, Hitchcock, P, Jackson, PJ, Keim, P, Kewalramani, AR, Longmire, J, Lucas, S, Malfatti, S, McMurry, K, Meincke, LJ, Misra, M, Moseman, BL, Mundt, M, Munk, AC, Okinaka, RT, Parson-Quintana, B, Reilly, LP, Richardson, P, Robinson, DL, Rubin, E, Saunders, E, Tapia, R, Tesmer, JG, Thayer, N, Thompson, LS, Tice, H, Ticknor, LO, Wills, PL, Brettin, TS & Gilna, P 2006, 'Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis', Journal of Bacteriology, vol. 188, no. 9, pp. 3382-3390. https://doi.org/10.1128/JB.188.9.3382-3390.2006

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title = "Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis",

abstract = "Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.",

author = "Han, {Cliff S.} and Gary Xie and Challacombe, {Jean F.} and Altherr, {Michael R.} and Bhotika, {Smriti S.} and David Bruce and Campbell, {Connie S.} and Campbell, {Mary L.} and Jin Chen and Olga Chertkov and Cathy Cleland and Mira Dimitrijevic and Doggett, {Norman A.} and Fawcett, {John J.} and Tijana Glavina and Goodwin, {Lynne A.} and Hill, {Karen K.} and Penny Hitchcock and Jackson, {Paul J.} and Paul Keim and Kewalramani, {Avinash Ramesh} and Jon Longmire and Susan Lucas and Stephanie Malfatti and Kim McMurry and Meincke, {Linda J.} and Monica Misra and Moseman, {Bernice L.} and Mark Mundt and Munk, {A. Christine} and Okinaka, {Richard T.} and B. Parson-Quintana and Reilly, {Lee Philip} and Paul Richardson and Robinson, {Donna L.} and Eddy Rubin and Elizabeth Saunders and Roxanne Tapia and Tesmer, {Judith G.} and Nina Thayer and Thompson, {Linda S.} and Hope Tice and Ticknor, {Lawrence O.} and Wills, {Patti L.} and Brettin, {Thomas S.} and Paul Gilna",

year = "2006",

month = may,

doi = "10.1128/JB.188.9.3382-3390.2006",

language = "English (US)",

volume = "188",

pages = "3382--3390",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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T1 - Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

AU - Han, Cliff S.

AU - Xie, Gary

AU - Challacombe, Jean F.

AU - Altherr, Michael R.

AU - Bhotika, Smriti S.

AU - Bruce, David

AU - Campbell, Connie S.

AU - Campbell, Mary L.

AU - Chen, Jin

AU - Chertkov, Olga

AU - Cleland, Cathy

AU - Dimitrijevic, Mira

AU - Doggett, Norman A.

AU - Fawcett, John J.

AU - Glavina, Tijana

AU - Goodwin, Lynne A.

AU - Hill, Karen K.

AU - Hitchcock, Penny

AU - Jackson, Paul J.

AU - Keim, Paul

AU - Kewalramani, Avinash Ramesh

AU - Longmire, Jon

AU - Lucas, Susan

AU - Malfatti, Stephanie

AU - McMurry, Kim

AU - Meincke, Linda J.

AU - Misra, Monica

AU - Moseman, Bernice L.

AU - Mundt, Mark

AU - Munk, A. Christine

AU - Okinaka, Richard T.

AU - Parson-Quintana, B.

AU - Reilly, Lee Philip

AU - Richardson, Paul

AU - Robinson, Donna L.

AU - Rubin, Eddy

AU - Saunders, Elizabeth

AU - Tapia, Roxanne

AU - Tesmer, Judith G.

AU - Thayer, Nina

AU - Thompson, Linda S.

AU - Tice, Hope

AU - Ticknor, Lawrence O.

AU - Wills, Patti L.

AU - Brettin, Thomas S.

AU - Gilna, Paul

PY - 2006/5

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N2 - Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

AB - Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

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JF - Journal of Bacteriology

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ER -

Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

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