Pathogen detection using single mode planar optical waveguides

Jennifer S. Martinez, W. Kevin Grace, Karen M. Grace, Nile Hartman, Basil I. Swanson

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

We utilize an optical waveguide-based biosensor recently developed at Los Alamos National Laboratory for the sensitive and specific detection of protein markers. Our planar optical waveguides are based on single mode structures that provide high optical field intensity at the active surface while providing discrimination from sample background fluorescence through spatial filtering. We have incorporated sandwich immunoassays where the capture antibody is immobilized on a biocompatible film attached to the surface of an optical waveguide. The approach can be adapted to any marker protein and is faster (<10 min), more sensitive, and as specific as conventional enzyme-linked immunosorbent assays (ELISA, the current lab standard). Results are presented for the detection of several analytes of Bacillus anthracis including protective antigen, a virulence marker for B. anthracis, and the bacterial cell or cellular debris.

Original languageEnglish (US)
Pages (from-to)4639-4647
Number of pages9
JournalJournal of Materials Chemistry
Volume15
Issue number43
DOIs
StatePublished - Nov 21 2005
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Pathogen detection using single mode planar optical waveguides'. Together they form a unique fingerprint.

Cite this