Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran

Emily Vogtmann; Yongli Han; J. Gregory Caporaso; Nicholas Bokulich; Ashraf Mohamadkhani; Alireza Moayyedkazemi; Xing Hua; Farin Kamangar; Yunhu Wan; Shalabh Suman; Bin Zhu; Amy Hutchinson; Casey Dagnall; Kristine Jones; Belynda Hicks; Jianxin Shi; Reza Malekzadeh; Christian C. Abnet; Akram Pourshams

doi:10.1002/cam4.2660

Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran

Emily Vogtmann, Yongli Han, J. Gregory Caporaso, Nicholas Bokulich, Ashraf Mohamadkhani, Alireza Moayyedkazemi, Xing Hua, Farin Kamangar, Yunhu Wan, Shalabh Suman, Bin Zhu, Amy Hutchinson, Casey Dagnall, Kristine Jones, Belynda Hicks, Jianxin Shi, Reza Malekzadeh, Christian C. Abnet, Akram Pourshams

Informatics, Computing, and Cyber Systems, School of

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Background: Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods: A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results: No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray-Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P-value of.000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion: The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies.

Original language	English (US)
Pages (from-to)	797-806
Number of pages	10
Journal	Cancer Medicine
Volume	9
Issue number	2
DOIs	https://doi.org/10.1002/cam4.2660
State	Published - Jan 1 2020

Keywords

case-control study
microbiota
pancreatic cancer

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1002/cam4.2660

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Vogtmann, E., Han, Y., Caporaso, J. G., Bokulich, N., Mohamadkhani, A., Moayyedkazemi, A., Hua, X., Kamangar, F., Wan, Y., Suman, S., Zhu, B., Hutchinson, A., Dagnall, C., Jones, K., Hicks, B., Shi, J., Malekzadeh, R., Abnet, C. C., & Pourshams, A. (2020). Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran. Cancer Medicine, 9(2), 797-806. https://doi.org/10.1002/cam4.2660

Vogtmann, E, Han, Y, Caporaso, JG, Bokulich, N, Mohamadkhani, A, Moayyedkazemi, A, Hua, X, Kamangar, F, Wan, Y, Suman, S, Zhu, B, Hutchinson, A, Dagnall, C, Jones, K, Hicks, B, Shi, J, Malekzadeh, R, Abnet, CC & Pourshams, A 2020, 'Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran', Cancer Medicine, vol. 9, no. 2, pp. 797-806. https://doi.org/10.1002/cam4.2660

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title = "Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran",

abstract = "Background: Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods: A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results: No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray-Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P-value of.000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion: The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies.",

keywords = "case-control study, microbiota, pancreatic cancer",

author = "Emily Vogtmann and Yongli Han and Caporaso, {J. Gregory} and Nicholas Bokulich and Ashraf Mohamadkhani and Alireza Moayyedkazemi and Xing Hua and Farin Kamangar and Yunhu Wan and Shalabh Suman and Bin Zhu and Amy Hutchinson and Casey Dagnall and Kristine Jones and Belynda Hicks and Jianxin Shi and Reza Malekzadeh and Abnet, {Christian C.} and Akram Pourshams",

note = "Publisher Copyright: Published 2019. This article is a U.S. Government work and is in the public domain in the USA. Cancer Medicine published by John Wiley & Sons Ltd.",

year = "2020",

month = jan,

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doi = "10.1002/cam4.2660",

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T1 - Oral microbial community composition is associated with pancreatic cancer

T2 - A case-control study in Iran

AU - Vogtmann, Emily

AU - Han, Yongli

AU - Caporaso, J. Gregory

AU - Bokulich, Nicholas

AU - Mohamadkhani, Ashraf

AU - Moayyedkazemi, Alireza

AU - Hua, Xing

AU - Kamangar, Farin

AU - Wan, Yunhu

AU - Suman, Shalabh

AU - Zhu, Bin

AU - Hutchinson, Amy

AU - Dagnall, Casey

AU - Jones, Kristine

AU - Hicks, Belynda

AU - Shi, Jianxin

AU - Malekzadeh, Reza

AU - Abnet, Christian C.

AU - Pourshams, Akram

N1 - Publisher Copyright: Published 2019. This article is a U.S. Government work and is in the public domain in the USA. Cancer Medicine published by John Wiley & Sons Ltd.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Background: Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods: A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results: No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray-Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P-value of.000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion: The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies.

AB - Background: Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods: A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results: No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray-Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P-value of.000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion: The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies.

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Oral microbial community composition is associated with pancreatic cancer: A case-control study in Iran

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