TY - JOUR
T1 - Next-generation sequencing of Coccidioides immitis isolated during cluster investigation
AU - Engelthaler, David M.
AU - Chiller, Tom
AU - Schupp, James A.
AU - Colvin, Joshua
AU - Beckstrom-Sternberg, Stephen M.
AU - Driebe, Elizabeth M.
AU - Moses, Tracy
AU - Tembe, Waibhav
AU - Sinari, Shripad
AU - Beckstrom-Sternberg, James S.
AU - Christoforides, Alexis
AU - Pearson, John V.
AU - Carpten, John
AU - Keim, Paul
AU - Peterson, Ashley
AU - Terashita, Dawn
AU - Arunmozhi Balajee, S.
PY - 2011/2
Y1 - 2011/2
N2 - Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 singlenucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
AB - Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 singlenucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
UR - http://www.scopus.com/inward/record.url?scp=79551636592&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79551636592&partnerID=8YFLogxK
U2 - 10.3201/eid1702.100620
DO - 10.3201/eid1702.100620
M3 - Article
C2 - 21291593
AN - SCOPUS:79551636592
SN - 1080-6040
VL - 17
SP - 227
EP - 232
JO - Emerging infectious diseases
JF - Emerging infectious diseases
IS - 2
ER -